Jiang Guangli, Qi Yuxia
Department of Obstetrics and Gynecology, Xinxiang Central Hospital, Xinxiang, Henan 453000, P.R. China.
Exp Ther Med. 2015 May;9(5):1939-1943. doi: 10.3892/etm.2015.2336. Epub 2015 Mar 9.
The aim of the present study was to investigate the correlation of matrix metalloproteinase (MMP)-9 and tissue inhibitor of matrix metalloproteinase inhibitor (TIMP)-3 expression with spontaneous abortion (SA) during early pregnancy. The villus tissues of 30 SA cases and 20 requested abortion cases were collected during surgery and constituted the SA and normal abortion (NA) groups, respectively. The total villous RNA was extracted and the expression levels of MMP -9 and TIMP-3 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR) assay to calculate the MMP-9/TIMP-3 mRNA ratio. The MMP-9 mRNA expression level and MMP-9/TIMP-3 mRNA ratio of the SA group were significantly higher than those of the NA group (P<0.01), while the TIMP-3 mRNA levels of the two groups were similar (P>0.05). The MMP-9 mRNA expression level of the SA group was higher than that of the NA group; thus, the MMP-9/TIMP-3 mRNA ratio was higher. These results suggest that the expression level of MMP-9 mRNA and the MMP-9/TIMP-3 mRNA ratio are associated with SA.
本研究的目的是探讨基质金属蛋白酶(MMP)-9和基质金属蛋白酶抑制剂(TIMP)-3的表达与早期妊娠自然流产(SA)的相关性。在手术过程中收集了30例自然流产病例和20例人工流产病例的绒毛组织,分别构成自然流产组和正常流产(NA)组。提取绒毛总RNA,采用逆转录-聚合酶链反应(RT-PCR)检测MMP-9和TIMP-3 mRNA的表达水平,计算MMP-9/TIMP-3 mRNA比值。自然流产组的MMP-9 mRNA表达水平和MMP-9/TIMP-3 mRNA比值显著高于正常流产组(P<0.01),而两组的TIMP-3 mRNA水平相似(P>0.05)。自然流产组的MMP-9 mRNA表达水平高于正常流产组;因此,MMP-9/TIMP-3 mRNA比值更高。这些结果表明,MMP-9 mRNA的表达水平和MMP-9/TIMP-3 mRNA比值与自然流产有关。