Reduced epithelial surface activity is related to a higher incidence of facial clefting in A/WySn mice.

The epithelial surfaces of the facial primordia were evaluated by scanning electron microscopy (SEM) during primary palatogenesis in two genetically related mouse strains, the A/J and the A/WySn strains. These two strains were selected because the reported frequency of spontaneous cleft lip with or without cleft palate [CL(P)] in the A/J strain approximates 0%, whereas the spontaneous frequency of CL(P) in the A/WySn strain is 20-30%. The embryos were examined prior to (two to six tail somites), during (seven to ten tail somites), and after (ten to 14 tail somites) primary palate fusion. During fusion, epithelial surface activity (characterized as cellular debris, dissociated cells, cellular projections, and epithelial bridging) was more pronounced in A/J embryos. A/WySn embryos with spontaneous cleft lip exhibited a marked deficiency in epithelial activity when compared to their normal littermates. No discernible differences were detected in the facial morphology, with the exception of the distal end of the medial nasal prominence, which appeared longer in the A/J strain. This study suggests that the degree of epithelial surface activity at the putative site of fusion and the relative length of the medial nasal prominence may account for the observed differences in facial clefting of the two strains. Face shape, related to prominence divergence, was similar in the two strains and could not explain the higher incidence of clefting observed in the A/WySn strain.