酵母 Hog1 MAP 激酶在应对渗透胁迫和衣霉素诱导的内质网应激时的核质活性。

Nuclear versus cytosolic activity of the yeast Hog1 MAP kinase in response to osmotic and tunicamycin-induced ER stress.

机构信息

Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Agustín Escardino, 7, 46980 Paterna, Valencia, Spain.

出版信息

FEBS Lett. 2015 Jul 22;589(16):2163-8. doi: 10.1016/j.febslet.2015.06.021. Epub 2015 Jun 30.

DOI:10.1016/j.febslet.2015.06.021

PMID:26140985

Abstract

We examined the physiological significance of the nuclear versus cytosolic localization of the MAPK Hog1p in the ability of yeast cells to cope with osmotic and ER (endoplasmic reticulum) stress. Our results indicate that nuclear import of Hog1p is not critical for osmoadaptation. Plasma membrane-anchored Hog1p is still able to induce increased expression of GPD1 and glycerol accumulation. This is a key osmoregulatory event, although a small production of the osmolyte coupled with the nuclear import of Hog1p is sufficient to provide osmoresistance. On the contrary, the nuclear activity of Hog1p is dispensable for ER stress adaptation.

摘要

我们研究了 MAPK Hog1p 的核质定位与酵母细胞应对渗透和 ER（内质网）应激能力的生理意义。结果表明，Hog1p 的核输入对于渗透适应并非关键。质膜锚定的 Hog1p 仍然能够诱导 GPD1 的表达增加和甘油积累。这是一个关键的渗透调节事件，尽管少量渗透物的产生与 Hog1p 的核输入相结合足以提供耐渗性。相反，Hog1p 的核活性对于 ER 应激适应是可有可无的。