López-Sanmartín Monserrat, Batista Frederico M, del Carmen Marín María, Garrido Inmaculada, Quintero David, Grade Ana, Ruano Francisco, de la Herrán Roberto, Navas José I
IFAPA Centro Agua del Pino, Consejería de Agricultura, Pesca y Desarrollo Rural, Junta de Andalucía, Ctra. El Rompido-Punta Umbría Km 3,8, 21459 Cartaya, Huelva, Spain.
Instituto Português do Mar e da Atmosfera, Divisão de Aquicultura e Valorização, Av. de Brasília, 1449-006 Lisboa, Portugal; Centro de Ciências do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
J Invertebr Pathol. 2015 Sep;130:52-5. doi: 10.1016/j.jip.2015.07.003. Epub 2015 Jul 3.
In the present study, Marteilia sp. was detected by histological examination and in situ hybridisation in Ostrea edulis and Ostrea stentina collected in southern Iberian Peninsula. Marteilia refringens DNA was detected by PCR in O. edulis (collected in southern Portugal) and O. stentina (collected in southern Spain and Portugal). Sequencing analysis revealed the presence of M. refringens type O in O. edulis, and type O and M in O. stentina. This is the first confirmed detection of M. refringens in Portugal and the first report on the occurrence of M. refringens infecting O. stentina in Europe.
在本研究中,通过组织学检查和原位杂交技术,在伊比利亚半岛南部采集的食用牡蛎和斯氏牡蛎中检测到马尔太虫属(Marteilia sp.)。通过聚合酶链反应(PCR)在葡萄牙南部采集的食用牡蛎和西班牙及葡萄牙南部采集的斯氏牡蛎中检测到了马尔太虫(Marteilia refringens)的DNA。测序分析显示,食用牡蛎中存在O型马尔太虫,斯氏牡蛎中存在O型和M型马尔太虫。这是葡萄牙首次确认检测到马尔太虫,也是欧洲首次报道马尔太虫感染斯氏牡蛎的情况。