Shandong Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases, Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong, Eye Institute of Shandong University of Traditional Chinese Medicine, Jinan, 250002, China.
Inflamm Res. 2015 Sep;64(9):683-96. doi: 10.1007/s00011-015-0848-3. Epub 2015 Jul 8.
We aimed to investigate the alterations of microRNA (miRNA) genomics in peripheral blood lymphocytes in experimental autoimmune uveitis (EAU) rats versus control samples.
MATERIALS/METHODS: Six Lewis rats received interphotoreceptor retinoid-binding protein (IRBP) emulsion to induce EAU. On day 12, peripheral blood lymphocytes were isolated, and total RNAs were extracted. Using microarray analysis, we analyzed the aberrant miRNAs, validated the relevant expression of differentially expressed miRNAs, and predicted the possible miRNA targets and signaling pathways.
The results indicated that 36 miRNAs were upregulated and 31 miRNAs were downregulated in EAU rats versus normal samples. Real-time quantitative PCR substantiated a high degree of confidence for the differentially expressed miRNAs, and miRNA analyses showed the differentially expressed miRNA targets were involved not only in the multicellular organismal process and developmental process, but also in T cell receptor signaling pathway, B cell receptor signaling pathway and so on.
Our findings show that the differentially expressed miRNAs in EAU rats were closely associated with immune signaling pathways and may be applied in early prevention, prognosis and possible therapy in uveitis, indicating that miRNAs play an important role in the development of uveitis.
我们旨在研究实验性自身免疫性葡萄膜炎（EAU）大鼠与对照样本相比外周血淋巴细胞中 microRNA（miRNA）基因组的改变。
材料/方法：六只 Lewis 大鼠接受光感受器间视网膜结合蛋白（IRBP）乳液以诱导 EAU。在第 12 天，分离外周血淋巴细胞并提取总 RNA。通过微阵列分析，我们分析了异常的 miRNA，验证了差异表达 miRNA 的相关表达，并预测了可能的 miRNA 靶标和信号通路。
结果表明，与正常样本相比，EAU 大鼠中有 36 个 miRNA 上调，31 个 miRNA 下调。实时定量 PCR 证实了差异表达 miRNA 的高度可信度，miRNA 分析表明，差异表达 miRNA 的靶标不仅参与了多细胞生物过程和发育过程，而且还参与了 T 细胞受体信号通路、B 细胞受体信号通路等。
我们的研究结果表明，EAU 大鼠中差异表达的 miRNA 与免疫信号通路密切相关，可能应用于葡萄膜炎的早期预防、预后和可能的治疗，表明 miRNA 在葡萄膜炎的发展中发挥重要作用。
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