猪感染H3N2型猪流感病毒和多杀性巴氏杆菌实验性共感染后的急性期蛋白反应概况
Profile of the porcine acute-phase proteins response following experimental co-infection with H3N2 swine influenza virus and Pasteurella multocida.
作者信息
Pomorska-Mól Małgorzata, Markowska-Daniel Iwona, Kwit Krzysztof, Stępniewska Katarzyna, Pejsak Zygmunt
机构信息
a Department of Swine Diseases , National Veterinary Research Institute , Pulawy , Poland.
出版信息
Biomarkers. 2015;20(3):189-95. doi: 10.3109/1354750X.2015.1061600. Epub 2015 Jul 13.
CONTEXT
Acute phase proteins (APPs) are proposed as potential markers of the health status in pigs.
OBJECTIVE
Circulating APPs in pigs co-infected with swine influenza virus and Pasteurella multocida.
METHODS
Serum APPs were measured in co-infected and control pigs with the use of commercial ELISA tests.
RESULTS
All investigated APPs revealed significant changes in co-infected pigs during the study period. The concentration of C-reactive protein, haptoglobin and serum amyloid A (SAA) increased significantly at 2 dpi, before respiratory signs and fever were observed. Concentration of Pig-MAP increased significantly at 3 dpi. C-reactive protein and SAA reaction were rapid but short-lived. The concentration of Hp and Pig-MAP in serum also increased at very early stage of co-infection but remained elevated for a longer period of time.
CONCLUSIONS
Maximal concentration of serum amyloid A correlated with the disease severity in pigs.
背景
急性期蛋白（APPs）被认为是猪健康状况的潜在标志物。
目的
研究感染猪流感病毒和多杀性巴氏杆菌的猪体内循环的APPs。
方法
使用商业ELISA检测法测定共感染猪和对照猪的血清APPs。
结果
在研究期间，所有检测的APPs在共感染猪中均显示出显著变化。在观察到呼吸道症状和发热之前，C反应蛋白、触珠蛋白和血清淀粉样蛋白A（SAA）的浓度在感染后2天显著升高。猪源主要急性期蛋白（Pig-MAP）的浓度在感染后3天显著升高。C反应蛋白和SAA反应迅速但持续时间短。在共感染的极早期，血清中触珠蛋白（Hp）和Pig-MAP的浓度也升高，但在较长时间内保持升高。
结论
血清淀粉样蛋白A的最大浓度与猪的疾病严重程度相关。
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