通过使用血清分离管、涂抹板制备和基质辅助激光解吸电离飞行时间质谱直接从阳性血培养物中快速鉴定细菌
Rapid Identification of Bacteria Directly from Positive Blood Cultures by Use of a Serum Separator Tube, Smudge Plate Preparation, and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.
作者信息
Chen Yan, Porter Vanessa, Mubareka Samira, Kotowich Leona, Simor Andrew E
机构信息
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
Department of Microbiology, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada.
出版信息
J Clin Microbiol. 2015 Oct;53(10):3349-52. doi: 10.1128/JCM.01493-15. Epub 2015 Jul 22.
Abstract
We analyzed the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) of smudge plate growth for bacterial identification from 400 blood cultures. Ninety-seven percent of Gram-negative bacilli and 85% of Gram-positive organisms were correctly identified within 4 h; only eight isolates (2.0%) were misidentified. This method provided rapid and accurate microbial identification from positive blood cultures.
摘要
我们分析了400份血培养物中用于细菌鉴定的涂抹平板生长的基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）。97%的革兰氏阴性杆菌和85%的革兰氏阳性菌在4小时内得到正确鉴定；只有8株分离菌（2.0%）被错误鉴定。该方法能从阳性血培养物中快速准确地鉴定微生物。
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