不同培养基中添加蛋白胨对CHO DG44细胞生长、代谢途径及生产力的影响；对氨基酸谱的新见解
Effects of Peptone Supplementation in Different Culture Media on Growth, Metabolic Pathway and Productivity of CHO DG44 Cells; a New Insight into Amino Acid Profiles.
作者信息
Davami Fatemeh, Eghbalpour Farnaz, Nematollahi Leila, Barkhordari Farzaneh, Mahboudi Fereidoun
机构信息
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Science and Research Branch, Islamic Azad University of Arak, Markazi Province, Iran.
出版信息
Iran Biomed J. 2015;19(4):194-205. doi: 10.7508/ibj.2015.04.002. Epub 2015 Aug 1.
BACKGROUND
The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development; however, their role on metabolic pathway is not well understood.
METHODS
In the present study, the effect of different concentration of peptones on a recombinant Chinese hamster ovary (CHO) cell line grown in three serum-free suspension cultures was determined. Six peptones of different origins and available amino acid profiles were investigated regarding their impact on cell growth, productivity, and metabolic pathways changes.
RESULTS
In optimized feeding strategies, increases of 136% and 159% in volumetric productivity (for a low-nutrient culture media) and 55% (for a high-nutrient culture media) were achieved. Furthermore, particular sources of peptones with specific amino acid profile developed preferential results for each different culture medium. Two peptones, SoyA2SC and SoyE-110, were the only hydrolysates that showed production improvement in all three media. Casein Peptone plus Tryptone N1 and SoyA3SC showed different improved results based on their implemented concentration for each individual basal medium.
CONCLUSION
The amino acid profile of peptones may provide clues to identify the most effective feeding strategies for recombinant CHO cells.
背景
在生物制药生产中，优化生物工艺条件以改善生长曲线和提高生产率产量被认为非常重要。蛋白胨作为高效营养源，其对培养基开发的影响已得到研究；然而，它们在代谢途径中的作用尚未得到充分理解。
方法
在本研究中，测定了不同浓度的蛋白胨对在三种无血清悬浮培养物中生长的重组中国仓鼠卵巢（CHO）细胞系的影响。研究了六种不同来源和氨基酸组成的蛋白胨对细胞生长、生产率和代谢途径变化的影响。
结果
在优化的补料策略中，（对于低营养培养基）体积生产率提高了136%和159%，（对于高营养培养基）提高了55%。此外，具有特定氨基酸组成的特定蛋白胨来源对每种不同的培养基产生了优先效果。两种蛋白胨，SoyA2SC和SoyE-110，是仅有的在所有三种培养基中都显示产量提高的水解产物。酪蛋白胨加胰蛋白胨N1和SoyA3SC根据其在每种基础培养基中的添加浓度显示出不同的改善结果。
结论
蛋白胨的氨基酸组成可能为确定重组CHO细胞最有效的补料策略提供线索。
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