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与辅酶A结合的GTP特异性琥珀酰辅酶A合成酶的结构

Structure of GTP-specific succinyl-CoA synthetase in complex with CoA.

作者信息

Huang Ji, Malhi Manpreet, Deneke Jan, Fraser Marie Elizabeth

机构信息

Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.

出版信息

Acta Crystallogr F Struct Biol Commun. 2015 Aug;71(Pt 8):1067-71. doi: 10.1107/S2053230X15011188. Epub 2015 Jul 29.

Abstract

Pig GTP-specific succinyl-CoA synthetase is an αβ-heterodimer. The crystal structure of the complex with the substrate CoA was determined at 2.1 Å resolution. The structure shows CoA bound to the amino-terminal domain of the α-subunit, with the free thiol extending from the adenine portion into the site where the catalytic histidine residue resides.

摘要

猪的GTP特异性琥珀酰辅酶A合成酶是一种αβ异二聚体。与底物辅酶A形成的复合物的晶体结构在2.1埃分辨率下得以确定。该结构显示辅酶A结合在α亚基的氨基末端结构域上,其游离硫醇从腺嘌呤部分延伸至催化组氨酸残基所在的位点。

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