Huang Ji, Malhi Manpreet, Deneke Jan, Fraser Marie Elizabeth
Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, Canada.
Acta Crystallogr F Struct Biol Commun. 2015 Aug;71(Pt 8):1067-71. doi: 10.1107/S2053230X15011188. Epub 2015 Jul 29.
Pig GTP-specific succinyl-CoA synthetase is an αβ-heterodimer. The crystal structure of the complex with the substrate CoA was determined at 2.1 Å resolution. The structure shows CoA bound to the amino-terminal domain of the α-subunit, with the free thiol extending from the adenine portion into the site where the catalytic histidine residue resides.
猪的GTP特异性琥珀酰辅酶A合成酶是一种αβ异二聚体。与底物辅酶A形成的复合物的晶体结构在2.1埃分辨率下得以确定。该结构显示辅酶A结合在α亚基的氨基末端结构域上,其游离硫醇从腺嘌呤部分延伸至催化组氨酸残基所在的位点。