Song D, Zhou X, Peng Q, Chen Y, Zhang F, Huang T, Zhang T, Li A, Huang D, Wu Q, He H, Tang Y
Jiangxi Agricultural University, Nanchang, Jiangxi, China.
Transbound Emerg Dis. 2015 Dec;62(6):575-80. doi: 10.1111/tbed.12399. Epub 2015 Aug 6.
To identify and characterize aetiologic agent(s) associated with an outbreak of a severe diarrhoea in piglets in Jiangxi, China, in March 2015, a nested reverse transcription-polymerase chain reaction (RT-PCR) for the detection of porcine deltacoronavirus (PDCoV) was developed. A survey based on the nested RT-PCR established indicated that the monoinfection of PDCoV (33.71%) and coinfection of PDCoV (19.66%) with porcine epidemic diarrhoea virus (PEDV) were common in diarrhoeal pigs in Jiangxi, China. A high prevalence of PDCoV (58.33%) in diarrhoeal samples which were PEDV negative was observed. The complete genome sequence of a representative PDCoV strain, PDCoV/CHJXNI2/2015, was determined. Phylogenetic analysis of complete genome and S protein sequences of PDCoV/CHJXNI2/2015 demonstrated that it was most closely related to Hong Kong and US PDCoVs. To our knowledge, this is the first report on the identification, prevalence, complete genome sequencing and molecular characterizations of PDCoV in diarrhoeal samples in pigs in China.
为鉴定和表征与2015年3月中国江西省仔猪严重腹泻暴发相关的病原体,开发了一种用于检测猪德尔塔冠状病毒(PDCoV)的巢式逆转录-聚合酶链反应(RT-PCR)。基于所建立的巢式RT-PCR进行的一项调查表明,在中国江西省腹泻猪中,PDCoV单一感染(33.71%)以及PDCoV与猪流行性腹泻病毒(PEDV)的混合感染(19.66%)很常见。在PEDV阴性的腹泻样本中观察到PDCoV的高流行率(58.33%)。测定了一株代表性PDCoV毒株PDCoV/CHJXNI2/2015的全基因组序列。对PDCoV/CHJXNI2/2015的全基因组和S蛋白序列进行的系统发育分析表明,它与香港和美国的PDCoV关系最为密切。据我们所知,这是关于中国猪腹泻样本中PDCoV的鉴定、流行率、全基因组测序和分子特征的首次报道。