含7-甲基鸟苷部分2'-和3'-羟基修饰的新型二核苷酸帽类似物的设计与简便合成
Design and Facile Synthesis of New Dinucleotide Cap Analog Containing Both 2' and 3'-OH Modification on M⁷Guanosine Moiety.
作者信息
Kore Anilkumar R, Bugarin Alejandro, Shanmugasundaram Muthian
机构信息
a Life Sciences Solutions Group, Thermo Fisher Scientific , Austin , Texas , USA.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2015;34(9):611-9. doi: 10.1080/15257770.2015.1041643.
The first example of the synthesis of new dinucleotide cap analog containing 2('),3(')-diacetyl group on m(7)guanosine moiety is described. The desired modified cap analog, m(7,2)(')(,3)(')(-diacetyl)G[5(')]ppp[5(')]G has been obtained by the coupling reaction of triethylamine salt of m(7,2)(')(,3)(')(-diacetyl)GDP with ImGMP in presence of ZnCl2 as a catalyst in 62% yield with high purity. The structure of new cap analog has been confirmed by (1)H and (31)P NMR and mass data.
描述了在7-甲基鸟苷部分合成含2′,3′-二乙酰基的新型二核苷酸帽类似物的首个实例。通过7,2′,3′-二乙酰基鸟苷二磷酸三乙胺盐与咪唑鸟苷5′-单磷酸在氯化锌作为催化剂存在下的偶联反应，以62%的产率和高纯度获得了所需的修饰帽类似物7,2′,3′-二乙酰基鸟苷5′-三磷酸5′-鸟苷（m(7,2)(')(,3)(')(-diacetyl)G[5(')]ppp[5(')]G）。新帽类似物的结构已通过氢核磁共振（¹H NMR）、磷-31核磁共振（³¹P NMR）和质谱数据得到证实。
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