中国耐多药结核病中赋予链霉素抗性的突变的鉴定
Identification of mutations conferring streptomycin resistance in multidrug-resistant tuberculosis of China.
作者信息
Zhao Li-Li, Liu Hai-Can, Sun Qing, Xiao Tong-Yang, Zhao Xiu-Qin, Li Gui-Lian, Zeng Chun-Yan, Wan Kang-Lin
机构信息
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, P.O. Box 5, Changping, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention/State Key Laboratory for Infectious Disease Prevention and Control, P.O. Box 5, Changping, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China; Pathogenic Biology Institute, University of South China, Hengyang 421001, Hunan Province, China.
出版信息
Diagn Microbiol Infect Dis. 2015 Oct;83(2):150-3. doi: 10.1016/j.diagmicrobio.2015.06.020. Epub 2015 Jul 2.
We investigated the spectrum and frequency of mutations in rpsL, rrs, and gidB among 140 multidrug-resistant tuberculosis (MDR-TB) clinical isolates from China. The association between mutations and different genotypes was also analyzed. Our data revealed that 65.7% of MDR-TB were resistant to streptomycin (STR), and 90.2% of STR-resistant isolates were Beijing strains. STR resistance was correlated with Beijing family (P=0.00). Compared with phenotypic data, detection of mutations for the combination of these 3 genes exhibited 94.6% sensitivity, 91.7% specificity, and 93.6% accuracy. The most common mutations in STR-resistant isolates were rpsL128, 262, and rrs514, of which rpsL128 showed association with Beijing lineage (P=0.00). A combination of these 3 mutations can serve as the reliable predictors for STR resistance, showing the sensitivity, specificity, and accuracy of 85.9%, 97.9%, and 90.0%, respectively. Furthermore, gidBA276C, not A615G, was Beijing lineage specific. These findings are useful to develop rapid molecular diagnostic methods for STR resistance in China.
我们调查了来自中国的140株耐多药结核病（MDR-TB）临床分离株中rpsL、rrs和gidB基因的突变谱及频率。同时分析了这些突变与不同基因型之间的关联。我们的数据显示，65.7%的MDR-TB对链霉素（STR）耐药，90.2%的STR耐药分离株为北京基因型菌株。STR耐药性与北京家族相关（P=0.00）。与表型数据相比，检测这3个基因的组合突变显示出94.6%的敏感性、91.7%的特异性和93.6%的准确性。STR耐药分离株中最常见的突变是rpsL128、262和rrs514，其中rpsL128与北京分支相关（P=0.00）。这3种突变的组合可作为STR耐药性的可靠预测指标，其敏感性、特异性和准确性分别为85.9%、97.9%和90.0%。此外，gidBA276C是北京分支特异性的，而非A615G。这些发现有助于在中国开发针对STR耐药性的快速分子诊断方法。
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