Preparation of red cell or saliva specific ABH antibodies To prepare agglutinins specific for red cell (anti-Hr, -Ar and -Br) or for saliva (anti-Hs, -As and -Bs), many animals were immunized with red cell or saliva. Then antisera were alternately absorbed with saliva or red cell. By these procedures, specific anti-Hr, -Ar, -Hs, -As and -Hs were prepared. Specificity of antisera to saliva was confirmed by latex agglutination which was adsorbed with saliva. 2. Quantitation of red cell or saliva specific antigen Quantitative analyses of Hs, As and Bs in saliva were performed by agglutination inhibition test, indicating that considerable individual difference in quantity of these antigens was observed. However, the difference was proved not to be genetically controlled. On the other hand, quantity of Hr or Ar antigens were distributed similarly among H, A, B and AB groups, or between A and AB groups, respectively. 3. Localization of Hr and Hs antigen in the salivary glands Distribution of the antigen was examined by ABC technique. Hs antigen was mainly located in the serous gland and slightly in the mucous gland and ductus. However, Hr was observed mainly in red cell and slightly in the ductus. H antigen detected with U. europaeus anti-H was distributed in all the glands. 4. Heterogeneity of Ulex europaeus anti-H (UE) UE is known to consist of two isolectins (UE-1 and UE-2) with different sugar specificity. Rabbit antiserum to UE-1 or UE-2 was prepared.(ABSTRACT TRUNCATED AT 250 WORDS)
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