中国某血液科分离出的肠球菌的分子特征及耐药谱
Molecular Characterization and Resistant Spectrum of Enterococci Isolated from a Haematology Unit in China.
作者信息
Yu Jiajia, Shi Jinfang, Zhao Ruike, Han Qingzhen, Qian Xuefeng, Gu Guohao, Zhang Xianfeng, Xu Jie
机构信息
Faculty, Department of Clinical Laboratory, The First Affiliated Hospital of Soochow University , Suzhou, P.R. of China .
出版信息
J Clin Diagn Res. 2015 Jun;9(6):DC04-7. doi: 10.7860/JCDR/2015/12864.6097. Epub 2015 Jun 1.
OBJECTIVES
The present study screened clinical isolates of E. faecalis and E. faecium to determine resistant spectrum and the potential virulence genes characterization among them of haematology patients.
METHODS
Clinical Enterococci isolates were obtained from a haematology unit in a tertiary care hospital in China.
RESULTS
Among 125 isolates available for the investigation, 46 were identified as E. faecium, and 79 were E. faecalis. Urine was the most common source (82, 65.6%). E. faecium isolates were more resistant than E. faecalis. Among E. faecium, maximum resistance was seen against PEN 93.5% and AMP 93.5% followed by CIP 87%. Eight vancomycin-resistant E. faecium (VREfm) isolates were obtained, positive for vanA genotype. Of 125 Enterococci isolates, 67(53.6%) were acm, and 42.4%, 25.6%, 25.6%, 24.8%, 23.2%, 20.8%, 10.4% and 7.2% of isolates were positive for esp, cylL-A, asa 1, cylL-S, cpd, cylL-L, gel-E and ace, respectively. E. faecalis isolates have more virulence genes (VGs) than E. faecium. MLST analysis of VREfm identified three different STs (ST17, ST78 and ST203).
CONCLUSION
The study provides the molecular characterization and resistant spectrum of Enterococci isolated from a haematology unit in China. Molecular analysis showed that all VREfm isolates belonged to pandemic clonal complex-17(CC17), associated with hospital-related isolates. Therefore, determining resistant spectrum and virulence characterization is crucial for the prevention and control of the spread of nosocomial infections caused by Enterococci in the haematology unit.
目的
本研究对粪肠球菌和屎肠球菌的临床分离株进行筛选，以确定血液科患者分离株的耐药谱及潜在毒力基因特征。
方法
临床肠球菌分离株来自中国一家三级医院的血液科。
结果
在可供调查的125株分离株中，46株被鉴定为屎肠球菌，79株为粪肠球菌。尿液是最常见的来源（82株，占65.6%）。屎肠球菌分离株比粪肠球菌耐药性更强。在屎肠球菌中，对青霉素的耐药率最高，为93.5%，氨苄西林为93.5%，其次是环丙沙星，为87%。获得8株耐万古霉素屎肠球菌（VREfm）分离株，vanA基因型呈阳性。在125株肠球菌分离株中，67株（53.6%）携带acm基因，分别有42.4%、25.6%、25.6%、24.8%、23.2%、20.8%、10.4%和7.2%的分离株esp、cylL-A、asa 1、cylL-S、cpd、cylL-L、gel-E和ace基因呈阳性。粪肠球菌分离株比屎肠球菌具有更多的毒力基因（VGs）。对VREfm进行多位点序列分型（MLST）分析，鉴定出三种不同的序列型（ST17、ST78和ST203）。
结论
本研究提供了从中国一家血液科分离的肠球菌的分子特征和耐药谱。分子分析表明，所有VREfm分离株均属于大流行克隆复合体-17（CC17），与医院相关分离株有关。因此，确定耐药谱和毒力特征对于预防和控制血液科由肠球菌引起的医院感染传播至关重要。
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