Turk J Med Sci. 2015;45(3):489-95. doi: 10.3906/sag-1404-5.
BACKGROUND/AIM: To evaluate the effects of grape seed extract (GSE) supplementation on oxidative stress and antioxidant markers in streptozotocin (STZ)-induced diabetic rats.
Thirty-six male rats were divided into the following four groups: control, GSE-supplemented control, diabetic, and GSE-supplemented diabetic. Beginning on day 7 after STZ injection, the rats were administered GSE (100 mg kg(-1) day(-1) in drinking water for 6 weeks. At the end of week 6, rats were sacrificed by cardiac puncture. Plasma nitric oxide (NO) levels and xanthine oxidase (XO), adenosine deaminase (ADA), and glutathione peroxidase (GPx) activities were analyzed.
Both XO and ADA activities increased and NO levels decreased in diabetic rats (P < 0.05). GSE supplementation normalized all of these changes. Antioxidant enzyme activities decreased in diabetic rats compared to the controls (P < 0.05). GSE supplementation increased antioxidant enzyme activities in both diabetic and healthy rats (P < 0.05).
These findings suggest that 6 weeks of oral GSE supplementation may prevent oxidative stress and improve antioxidant status in diabetic rats.
背景/目的：评估葡萄籽提取物（GSE）补充对链脲佐菌素（STZ）诱导的糖尿病大鼠氧化应激和抗氧化标志物的影响。
36 只雄性大鼠被分为以下四组：对照组、GSE 补充对照组、糖尿病组和 GSE 补充糖尿病组。自 STZ 注射后第 7 天开始，大鼠每天通过饮用水给予 GSE（100mgkg(-1)），持续 6 周。在第 6 周末，通过心脏穿刺处死大鼠。分析血浆中一氧化氮（NO）水平以及黄嘌呤氧化酶（XO）、腺苷脱氨酶（ADA）和谷胱甘肽过氧化物酶（GPx）的活性。
与对照组相比，糖尿病大鼠的 XO 和 ADA 活性增加，NO 水平降低（P<0.05）。GSE 补充使所有这些变化都恢复正常。与对照组相比，糖尿病大鼠的抗氧化酶活性降低（P<0.05）。GSE 补充增加了糖尿病和健康大鼠的抗氧化酶活性（P<0.05）。
这些发现表明，口服 GSE 补充 6 周可能预防糖尿病大鼠的氧化应激并改善其抗氧化状态。
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