Zheng Shao-qiang, Chen Xue, Wang Ya-jie, An Li-xin
Department of Anesthesiology, Beijing Jishuitan Hospital, Beijing 100035, China.
Department of Clinical Laboratory, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2015 Aug 18;47(4):674-8.
To investigate the changes of neuroapoptosis in brain and learning ability after neonatal mice are exposed to inhaled sevoflurane.
Twenty-one postnatal day (P) 7 Wistar rats were randomly divided into 7 groups for the preliminary experiment. Arterial blood samples were obtained immediately at the end of anesthesia, then blood analysis was performed. According to the results of the blood analysis, the groups that had no carbon dioxide accumulation were chosen for the following experiment. Ninety postnatal day (P) 7 Wistar rats were randomly divided into 5 groups: group A [sham anesthesia], group B [1% (volume fraction) sevoflurane for 2 h], group C (1% sevoflurane for 4 h), group D [2% (volume fraction) sevoflurane for 2 h] and group E (2% sevoflurane for 4 h). The animals from each group were perfused transcardially with 0.1 mol/L phosphate buffer containing 4% (volume fraction) paraformaldehyde 6 h after the end of anesthesia, and then the brains were exposed for immunohisochemistry, and caspase-3 positive cells were detected. Behavioral studies which included Morris water maze and passive voidance test were performed separately when the rats were 5-week-old, 8-week-old and 14-week-old.
The blood gas data in the mice during the anesthesia showed that the pH, arterial carbon dioxide tension, arterial oxygen tension, and arterial oxygen saturation did not differ significantly from those of the sham controls. The amount of the caspase-3 positive cells in the rat brains of group B, group D and group E was greater than that in group A. When facing the spatial reference memory task or space exploration task, the rats from the different groups made it uniformly. The rats exposed to sham anesthesia had longer latency and less mistake times than those to sevoflurane in passive voidance test when they were 5-week-old, while all the rats had no significant difference in 8 weeks.
Exposure to the concentration of 2% sevoflurane causes brain cell apoptosis of newborn rats. The memory ability to pessimal stimulation is decreased as the anesthesia mice were 5-week-old, such changes recede along with the growth of the rats. Exposure to the concentration of 2% sevoflurane does not affect the spatial reference memory of newborn rats during their growth.
探讨新生小鼠吸入七氟醚后大脑神经细胞凋亡及学习能力的变化。
将21只出生后7天（P7）的Wistar大鼠随机分为7组进行预实验。麻醉结束后立即采集动脉血样本，随后进行血液分析。根据血液分析结果，选择无二氧化碳蓄积的组进行后续实验。将90只出生后7天（P7）的Wistar大鼠随机分为5组：A组[假麻醉]、B组[1%（体积分数）七氟醚，2小时]、C组（1%七氟醚，4小时）、D组[2%（体积分数）七氟醚，2小时]和E组（2%七氟醚，4小时）。麻醉结束6小时后，对每组动物进行经心灌注含4%（体积分数）多聚甲醛的0.1mol/L磷酸盐缓冲液，然后取出大脑进行免疫组织化学检测，检测caspase-3阳性细胞。当大鼠5周龄、8周龄和14周龄时，分别进行包括Morris水迷宫和被动回避试验在内的行为学研究。
麻醉期间小鼠的血气数据显示，pH值、动脉二氧化碳分压、动脉氧分压和动脉血氧饱和度与假手术对照组相比无显著差异。B组、D组和E组大鼠脑内caspase-3阳性细胞数量多于A组。面对空间参考记忆任务或空间探索任务时，不同组大鼠表现一致。5周龄时，假麻醉组大鼠在被动回避试验中的潜伏期比七氟醚麻醉组大鼠长，错误次数比七氟醚麻醉组大鼠少，而8周龄时所有大鼠无显著差异。
吸入2%七氟醚可导致新生大鼠脑细胞凋亡。5周龄麻醉小鼠对不良刺激的记忆能力下降，随着大鼠生长，这种变化逐渐消退。吸入2%七氟醚不影响新生大鼠生长过程中的空间参考记忆。
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