Zhao Yue'e, Lei Wensheng
Department of Ophthalmology, Lishui Central Hospital, the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, China.
Department of Ophthalmology, Lishui Central Hospital, the Fifth Affiliated Hospital of Wenzhou Medical University, Lishui 323000, China; Email:
Zhonghua Yan Ke Za Zhi. 2015 May;51(5):356-9.
To investigate whether ranibizumab can penetrate into the retina after subconjunctival injection in a rabbit model.
Experimental study. Fifty-six New Zealand rabbits were randomly divided into four groups: control group (3), saline subconjunctival injection group (3), ranibizumab intravitreal injection group (25) and ranibizumab subconjunctival injection group (25). Each rabbit received single injection in right eye. Ranibizumab's location and concentrations in the retina and aqueous humor were determined by immunofluorescence staining and ELISA at 1, 2, 4, 8, 12 weeks after injection. The comparison of ranibizumab concentration data was analyzed by t-test.
The concentrations of aqueous humor in intravitreal injection group, determined by ELISA, were (389.67 ± 22.55), (319.33 ± 17.93), (22.67 ± 3.06), (9.67 ± 1.53) and (7.33 ± 2.18) mg/L at 1, 2, 4, 8, 12 weeks respectively. Concentrations of subconjunctival injection group were (53.67 ± 3.21), (12.67 ± 3.06), (8.33 ± 1.53), (2.66 ± 2.08) mg/L at 1,2,4,8 weeks and had a statistical difference compared with intravitreal injection group at each time point (t = 32.96, 38.39, 7.67, 11.07, P < 0.05). But there was no detection at 12 weeks. Similar in retina immunofluorescence staining results, the ranibizumab positive signals were detected in all the time points except the last one in subconjunctival injection group.
Subconjunctival ranibizumab injection may be able to penetrate into retina.
在兔模型中研究结膜下注射雷珠单抗后其能否穿透进入视网膜。
实验研究。56只新西兰兔随机分为四组：对照组（3只）、结膜下注射生理盐水组（3只）、玻璃体内注射雷珠单抗组（25只）和结膜下注射雷珠单抗组（25只）。每只兔右眼接受单次注射。在注射后1、2、4、8、12周，通过免疫荧光染色和酶联免疫吸附测定法（ELISA）测定视网膜和房水中雷珠单抗的位置和浓度。雷珠单抗浓度数据的比较采用t检验分析。
ELISA测定玻璃体内注射组房水浓度在1、2、4、8、12周分别为（389.67±22.55）、（319.33±17.93）、（22.67±3.06）、（9.67±1.53）和（7.33±2.18）mg/L。结膜下注射组在1、2、4、8周的浓度分别为（53.67±3.21）、（12.67±3.06）、（8.33±1.53）、（2.66±2.08）mg/L，在各时间点与玻璃体内注射组相比有统计学差异（t = 32.96、38.39、7.67、11.07，P < 0.05）。但在12周未检测到。视网膜免疫荧光染色结果类似，结膜下注射组除最后一个时间点外，在所有时间点均检测到雷珠单抗阳性信号。
结膜下注射雷珠单抗可能能够穿透进入视网膜。
