[In vivo Cell CFSE Fluorescence Negative Staining for Detection of Super Paramagnetic Iron Oxide Nanoparticles Phagocytosed by Mouse Mononuclear Macrophage Leukemia Cells-RAW264.7].
作者信息
He Xiang-Feng, Yang Li-Ping, Chen Bao-An, Wang Jian-Hong, Wen Song, Shi Wen, Xu Wei-Wei, Ling Guo-Jie
机构信息
Department of Hematology (Key Discipline of Medicine, Jiangsu Province), Zhongda Hospital of Southeast University, Nanjing 210009, Jiangsu Province, China.
Department of Internal Medicine, Nantong Cancer Hospital Affiliated to Nantong University, Nantong 226361, Jiangsu Province, China.
出版信息
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2015 Aug;23(4):1168-72. doi: 10.7534/j.issn.1009-2137.2015.04.051.
OBJECTIVE
To explore the feasibility and fluorescence characteristics of CFSE negative staining for in vivo cell imaging of super paramagnetic iron oxide nanoparticles (SPIO) phagocytosed by mouse mononuclear macrophage leukemia cells-RAW264.7.
METHODS
After labeled with SPIO, the RAW264.7 macrophages were stained with Prussian blue stain and CFSE fluorescence negative stain step by step. Furthermore, trypan blue staining was used to evaluate cell viability of cells which stained with CFSE. At last, laser scanning confocal microscope was used to measure SPIO in cells through CFSE fluorescence negative stain method.
RESULTS
SPIO within RAW264.7 macrophages showed blue in Prussian's blue staining, while showed negative area in CFSE negative staining. Good consistencies between Prussian's blue staining and CFSE negative staining were observed. In addition, RAW264.7 macrophages showed high viability after SPIO/CFSE dual-labeled method, proved by typan stain.
CONCLUSION
The CFSE fluorescence negative staining may be used for detecting SPIO that phagocytosed by RAW264.7 macrophages and it is showed good consistency that confirmed one another when compared to classic Prussian' blue staining.
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