Veryser Lieselotte, Taevernier Lien, Roche Nathalie, Blondeel Phillip, De Spiegeleer Bart
Drug Quality and Registration (DruQuaR) group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, Belgium.
Department of Plastic and Reconstructive Surgery, University Hospital Ghent, De Pintelaan 185, B-9000 Ghent, Belgium.
J Pharm Biomed Anal. 2015 Nov 10;115:594-602. doi: 10.1016/j.jpba.2015.08.016. Epub 2015 Aug 18.
In this study, a new type of single quadrupole mass spectrometric detector was implemented in transdermal research. The local skin pharmacokinetic properties of the plant N-alkylamides (NAAs) pellitorine and anacycline, present in an Anacyclus pyrethrum extract, and spilanthol, present in a Spilanthes acmella extract were investigated. This single quad MS detection method showed great advantages compared to the traditional UV detector. The NAAs could be identified and quantified in the samples with an ultra performance liquid chromatography (UPLC)-single quad MS detection system, even if they were not separated, which is a requirement when using an UV-detector. Another advantage of the UPLC-MS system is that lower limit of detection values could be obtained allowing a more accurate and precise determination of the experimental lag time in the in vitro skin permeation experiments. To conclude, this single quad MS detector coupled to UPLC is a useful analytical tool with improved performance compared to high performance liquid chromatography (HPLC)-UV for biomedical-pharmaceutical purposes in transdermal research.
在本研究中，一种新型单四极杆质谱检测器被应用于透皮研究。对存在于洋甘菊提取物中的植物N-烷基酰胺（NAAs）派立托宁和去甲山油柑碱，以及存在于刺苞菜提取物中的毛果芸香碱的局部皮肤药代动力学特性进行了研究。与传统紫外检测器相比，这种单四极杆质谱检测方法显示出巨大优势。即使NAAs未被分离，也可通过超高效液相色谱（UPLC）-单四极杆质谱检测系统在样品中对其进行鉴定和定量，而这是使用紫外检测器时的一项要求。UPLC-MS系统的另一个优势是能够获得更低的检测限，从而在体外皮肤渗透实验中更准确、精确地测定实验滞后时间。总之，与高效液相色谱（HPLC）-紫外检测器相比，这种与UPLC联用的单四极杆质谱检测器是一种性能更优的有用分析工具，可用于透皮研究中的生物医学-制药目的。
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