Wang Xiaohui, Che Honglei, Zhang Wenbin, Wang Jiye, Ke Tao, Cao Rui, Meng Shanshan, Li Dan, Weiming Ouyang, Chen Jingyuan, Luo Wenjing
1. Department of Occupational & Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi'an 710032, China.
2. Immunology Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10065, USA.
Int J Biol Sci. 2015 Aug 8;11(10):1171-80. doi: 10.7150/ijbs.12161. eCollection 2015.
Cold adaptation is a body's protective response to cold stress. Mild chronic intermittent cold (CIC) exposure has been used to generate animal models for cold adaptation studies. However, the effects of mild CIC exposure on vital organs are not completely characterized. In the present study, we exposed rats to mild CIC for two weeks, and then measured the body weights, the weights of brown adipose tissue (BAT), the levels of ATP and reactive oxygen species (ROS) in the brains, livers, hearts, muscles and BATs. Rats formed cold adaptation after exposure to CIC for two weeks. Compared to rats of the control group that were hosted under ambient temperature, rats exposed to mild CIC showed a lower average body weight, but a higher weight of brown adipose tissue (BAT). Rats exposed to CIC for two weeks also exhibited higher levels of ATP and ROS in all examined organs as compared to those of the control group. In addition, we determined the expression levels of cold-inducible RNA binding protein (Cirbp) and thioredoxin (TRX) in rat tissues after 2 weeks of CIC exposure. Both Cirbp and TRX were increased, suggesting a role of these two proteins for establishment of cold adaptation. Together, this study reveals the effects of mild CIC exposure on vital organs of rats during CIC exposure.
冷适应是机体对冷应激的一种保护性反应。轻度慢性间歇性冷暴露（CIC）已被用于建立冷适应研究的动物模型。然而，轻度CIC暴露对重要器官的影响尚未完全明确。在本研究中，我们将大鼠暴露于轻度CIC环境两周，然后测量其体重、棕色脂肪组织（BAT）重量、脑、肝、心、肌肉和BAT中的ATP水平及活性氧（ROS）水平。大鼠在暴露于CIC两周后形成冷适应。与饲养在环境温度下的对照组大鼠相比，暴露于轻度CIC的大鼠平均体重较低，但棕色脂肪组织（BAT）重量较高。与对照组相比，暴露于CIC两周的大鼠在所有检测器官中也表现出较高的ATP和ROS水平。此外，我们测定了CIC暴露2周后大鼠组织中冷诱导RNA结合蛋白（Cirbp）和硫氧还蛋白（TRX）的表达水平。Cirbp和TRX均升高，表明这两种蛋白在冷适应的建立中发挥作用。总之，本研究揭示了轻度CIC暴露在CIC暴露期间对大鼠重要器官的影响。
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