Vorovich M F, Timofeev A V, Mal'dov D G, Khaustov V I, El'bert L B
Vopr Virusol. 1989 Nov-Dec;34(6):698-701.
The study showed the disruption of disulphide bonds in E protein of tick-borne encephalitis virus (TBE) to lead to the loss of antigenicity, infectivity, hemagglutinating and protective activities. The loss of infectivity under the effect of a thiolic reagent appears to be associated with block of the very first stage of virus-cell interaction, virus adsorption on the target cell. An attempt to reestablish the E protein structure and the above-mentioned virus properties after the removal of the thiolic reagent failed. The role of tertiary structure of E protein in the manifestation of TBE virus main biological properties is discussed.
研究表明，蜱传脑炎病毒（TBE）E蛋白中的二硫键断裂会导致抗原性、感染性、血凝活性和保护活性丧失。硫醇试剂作用下感染性的丧失似乎与病毒-细胞相互作用的最初阶段受阻，即病毒吸附在靶细胞上有关。去除硫醇试剂后试图重建E蛋白结构和上述病毒特性的尝试失败了。文中讨论了E蛋白三级结构在蜱传脑炎病毒主要生物学特性表现中的作用。
