Chu Qing, Gao Yunhang, Xu Guoliang, Wu Changwen, Xu Tianjun
Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022 China.
College of Animal Science and Veterinary Medicine, Jilin Agriculture University, Changchun, 130118 China.
Fish Shellfish Immunol. 2015 Nov;47(1):168-74. doi: 10.1016/j.fsi.2015.08.032. Epub 2015 Aug 31.
Miiuy croker (Miichthys miiuy) as an important economical aquaculture species has challenged many more diseases caused by various pathogens recently. To better explore the immune response to virus, we have analyzed the transcriptome profiling of miiuy croaker challenged with poly(I:C) synthetic analog of virus dsRNA. We have obtained differentially expressed genes (DEGs) with up/down-relevant from comparison of the Ctrl and Poly transcriptome libraries. Through GO and KEGG enrichment analysis, immune-relevant DEGs whose expression are significantly rise or fall after challenged have been identified and classified. In order to detailedly analysis host immune response patterns for dsRNA virus, we have performed a map based on RIG-I/MDA5-mediated and TLR3-mediated signaling pathway which both induced type I IFNs response. In this pathway, both MDA5 and LGP2 are important RLRs in host surveillance against infection of dsRNA viruses and induce type I IFNs response which subsequently form a transcription factor complex ISGF3 that promote downstream genes referred to as ISGs to inhibits virus replication.
大黄鱼（Miichthys miiuy）作为一种重要的经济水产养殖品种，近年来受到多种病原体引发的更多疾病的挑战。为了更好地探索对病毒的免疫反应，我们分析了用病毒双链RNA的聚肌胞苷酸（poly(I:C)）合成类似物刺激的大黄鱼的转录组图谱。通过比较对照和聚肌胞苷酸转录组文库，我们获得了上调/下调相关的差异表达基因（DEG）。通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析，已鉴定并分类了在刺激后表达显著上升或下降的免疫相关DEG。为了详细分析宿主对双链RNA病毒的免疫反应模式，我们基于视黄酸诱导基因I（RIG-I）/黑色素瘤分化相关蛋白5（MDA5）介导的和Toll样受体3（TLR3）介导的信号通路绘制了图谱，这两条通路均诱导I型干扰素反应。在该通路中，MDA5和实验室遗传学与生理学2（LGP2）都是宿主监测双链RNA病毒感染的重要视黄酸诱导基因样受体（RLR），并诱导I型干扰素反应，随后形成转录因子复合物干扰素刺激基因因子3（ISGF3），促进称为干扰素刺激基因（ISG）的下游基因抑制病毒复制。
