焦磷酸镁在聚合酶链式反应（PCR）过程中含DNA微粒形成中的结构形成作用。
The structure-forming role of magnesium pyrophosphate in the formation of DNA-containing microparticles during PCR.
作者信息
Danilevich V N, Machulin A V, Sorokin V V, Mulyukin A L
机构信息
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia,
出版信息
Dokl Biochem Biophys. 2015;463:259-63. doi: 10.1134/S1607672915040171. Epub 2015 Sep 3.
PMID:26335826
Abstract
This work is devoted to studying the mechanisms of formation of DNA-containing microparticles (MPs) during PCR. It was found that pyrophosphate, a byproduct of DNA synthesis, and magnesium cations are required for their formation, as evidenced by the results of biochemical and electron microscopy studies.
摘要
这项工作致力于研究聚合酶链反应（PCR）过程中含DNA微粒（MPs）的形成机制。生化和电子显微镜研究结果表明，DNA合成的副产物焦磷酸和镁离子是其形成所必需的。
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