一种测定水相中叠氮化物和氰化物的酶法。
An enzymatic method for determination of azide and cyanide in aqueous phase.
作者信息
Wan Nan-Wei, Liu Zhi-Qiang, Xue Feng, Zheng Yu-Guo
机构信息
Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, PR China.
Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China; Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou 310014, PR China.
出版信息
J Biotechnol. 2015 Nov 20;214:27-32. doi: 10.1016/j.jbiotec.2015.09.001. Epub 2015 Sep 7.
A halohydrin dehalogenase (HHDH-PL) from Parvibaculum lavamentivorans DS-1 was characterized and applied to determine azide and cyanide in the water. In this methodology, HHDH-PL catalysed azide and cyanide to react with butylene oxide and form corresponding β-substituted alcohols 1-azidobutan-2-ol (ABO) and 3-hydroxypentanenitrile (HPN) that could be quantitatively detected by gas chromatograph. The detection calibration curves for azide (R(2)=0.997) and cyanide (R(2)=0.995) were linear and the lower limits of detection for azide and cyanide were 0.1 and 0.3mM, respectively. Several other nucleophiles were identified having no effect on the analysis of azide and cyanide, excepting nitrite which influenced the detection of cyanide. This was the first report of a biological method to determine the inorganic azide and cyanide by converting them to the measurable organics.
对来自食污小杆菌DS-1的卤代醇脱卤酶（HHDH-PL）进行了表征，并将其用于测定水中的叠氮化物和氰化物。在此方法中，HHDH-PL催化叠氮化物和氰化物与环氧丁烷反应，形成相应的β-取代醇1-叠氮基丁-2-醇（ABO）和3-羟基戊腈（HPN），这些产物可通过气相色谱法定量检测。叠氮化物（R² = 0.997）和氰化物（R² = 0.995）的检测校准曲线呈线性，叠氮化物和氰化物的检测下限分别为0.1和0.3 mM。除了影响氰化物检测的亚硝酸盐外，还鉴定出其他几种亲核试剂对叠氮化物和氰化物的分析没有影响。这是关于通过将无机叠氮化物和氰化物转化为可测量的有机物来测定它们的生物学方法的首次报道。
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