一种基于固定化酶技术的用于生产L-2-氨基丁酸的可循环生物转化系统。
A recyclable biotransformation system for L-2-aminobutyric acid production based on immobilized enzyme technology.
作者信息
Xu Gang, Jiang Yu, Tao Rongshen, Wang Shengfeng, Zeng Hongyu, Yang Sheng
机构信息
College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, 410004, China.
Hunan Flag Bio-tech Co. Ltd, Changsha, 410100, China.
出版信息
Biotechnol Lett. 2016 Jan;38(1):123-9. doi: 10.1007/s10529-015-1957-3. Epub 2015 Sep 16.
OBJECTIVE
To make the previously developed biosynthesis of L-2-aminobutyric acid (L-ABA) more suitable for the industrial-scale production.
RESULTS
A recyclable biotransformation system was developed based on immobilized enzyme technology. The conversion yield of L-threonine (at 90 g l(-1)) reached 99.9 % and the theoretical yield of L-ABA reached more than 90 % using the optimized biotransformation system by the individual immobilization of threonine deaminase and the co-immobilization of L leucine dehydrogenase and formate dehydrogenase. 90 g L-threonine l(-1) was converted to 73.9 g L-ABA l(-1) >95 % theoretical yield, within 120-145 min in 30 batch transformation experiments.
CONCLUSION
The recyclable biotransformation system is promising to fulfill industrial requirements for L-ABA production.
目的
使先前开发的L-2-氨基丁酸（L-ABA）生物合成方法更适合工业规模生产。
结果
基于固定化酶技术开发了一种可循环生物转化系统。通过单独固定苏氨酸脱氨酶以及共固定L-亮氨酸脱氢酶和甲酸脱氢酶，利用优化后的生物转化系统，L-苏氨酸（90 g l(-1)）的转化率达到99.9%，L-ABA的理论产率超过90%。在30批次转化实验中，90 g L-苏氨酸l(-1)在120 - 145分钟内转化为73.9 g L-ABA l(-1)，理论产率>95%。
结论
该可循环生物转化系统有望满足L-ABA生产的工业需求。
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