Surfactant-Free Emulsion-Based Preparation of Redox-Responsive Nanogels.

A surfactant-free emulsion-based approach is developed for preparation of nanogels. A water-in-oil emulsion is generated feasibly from a mixture of water and a solution of disulfide-containing hyperbranched PEGylated poly(amido amine)s, poly(BAC2-AMPD1)-PEG, in chloroform. The water droplets in the emulsion are stabilized and filled with poly(BAC2-AMPD1)-PEG, and the crosslinked poly(amido amine)s nanogels are formed via the intermolecular disulfide exchange reaction. FITC-dextran is loaded within the nanogels by dissolving the compound in water before emulsification. Transmission electron microscopy and dynamic light scattering are applied to characterize the emulsion and the nanogels. The effects of the homogenization rate and the ratio of water/polymer are investigated. Redox-induced degradation and FITC-dextran release profile of the nanogels are monitored, and the results show efficient loading and redox-responsive release of FITC-dextran. This is a promising approach for the preparation of nanogels for drug delivery, especially for neutral charged carbohydrate-based drugs.