缺氧诱导脐带血间充质干细胞分化为胰岛素分泌细胞的机制研究
Mechanism study for hypoxia induced differentiation of insulin-producing cells from umbilical cord blood-derived mesenchymal stem cells.
作者信息
Sun Bo, Meng Xian-Hui, Liu Rui, Yan Shancheng, Xiao Zhong-Dang
机构信息
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; Institute of Microbiology, Seoul National University, Seoul 151-742, South Korea.
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
出版信息
Biochem Biophys Res Commun. 2015 Oct 23;466(3):444-9. doi: 10.1016/j.bbrc.2015.09.047. Epub 2015 Sep 25.
Recently, we have successfully obtained functional IPCs efficiently from umbilical cord blood-derived mesenchymal stem cells by using hypoxia treatment. In this study, we further elaborated that the improved function and viability of IPCs are the result of the interaction β cell development pathway and c-Met/HGF axis induced by hypoxia. We found that hypoxia induced c-MET elevation is efficiently initiated the early stage differentiation IPCs from MSCs, and HGF improved the fully differentiation of IPCs by inducing the expression of NGN3. This finding may contribute to understanding β cell development and the development of stem cell therapy for diabetes.
最近，我们通过缺氧处理成功地从脐带血来源的间充质干细胞中高效获得了功能性胰岛祖细胞（IPCs）。在本研究中，我们进一步阐述了IPCs功能和活力的改善是缺氧诱导的β细胞发育途径与c-Met/肝细胞生长因子（HGF）轴相互作用的结果。我们发现，缺氧诱导的c-MET升高有效地启动了间充质干细胞向IPCs的早期分化，而HGF通过诱导神经生成蛋白3（NGN3）的表达促进了IPCs的完全分化。这一发现可能有助于理解β细胞发育以及糖尿病干细胞治疗的发展。
Zotero 插件