马铃薯M病毒富含半胱氨酸蛋白的分子变异性
Molecular variability in the cysteine rich protein of potato virus M.
作者信息
Tabasinejad Fatemeh, Jafarpour Behrooz, Zakiaghl Mohammad, Siampour Majid, Rowhani Hamid, Mehrvar Mohsen
机构信息
Department of Plant Pathology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Plant Protection, College of Agriculture, Shahrekord University, Shahrekord, Iran.
出版信息
Virusdisease. 2015 Sep;26(3):117-22. doi: 10.1007/s13337-015-0258-8. Epub 2015 Jun 26.
The potato virus M (PVM), belonging to the genus Carlavirus, is a worldwide endemic pathogen in potato fields. p11 is an 11-16 kDa protein encoded by the last open reading frame of PVM which contains cysteine rich proteins (CRPs) motif. CRPs have been identified as suppressors of gene silencing. In this study the p11 gene from 28 PVM isolates, including 16 new isolates from Iran, were used to determine the global genetic structure of PVM populations. Pairwise nucleotide sequence identity scores showed that global PVM CRP sequence similarity was between 69.3 and 100 %. This genetic diversity divided the 28 isolates into two main divergent phylogenetic clades. The rate of genetic diversity and non-synonymous to synonymous mutations (dN/dS) were significantly different between these two clades. Analysis showed that PVM CP is under significant negative selection pressure with the global ω value of 0.260.
马铃薯病毒M（PVM）属于香石竹潜隐病毒属，是一种在全球马铃薯种植田中普遍存在的病原体。p11是由PVM最后一个开放阅读框编码的一种11 - 16 kDa的蛋白质，其含有富含半胱氨酸的蛋白质（CRP）基序。CRP已被确定为基因沉默的抑制因子。在本研究中，来自28个PVM分离株（包括来自伊朗的16个新分离株）的p11基因被用于确定PVM群体的全球遗传结构。成对核苷酸序列同一性得分表明，全球PVM CRP序列相似性在69.3%至100%之间。这种遗传多样性将28个分离株分为两个主要的不同系统发育分支。这两个分支之间的遗传多样性速率以及非同义突变与同义突变的比率（dN/dS）存在显著差异。分析表明，PVM外壳蛋白（CP）处于显著的负选择压力之下，全球ω值为0.260。
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