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光声光谱法在蜂蜜质量鉴定中的应用

The Application of Quality Identification in Honey by Photoacoustic Spectroscopy.

作者信息

Tao Wen-ting, Yuan Ping, Guo Wen-juan, Liu Jian-en

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2015 May;35(5):1177-81.

Abstract

The photoacoustic spectrum of glucose, sucrose and honey solutions in the visible range are measured by using the single-light photoacoustic spectrometer, and are compared with the spectra from spedtrophotometry method. The spectral characteristics of the above solutions show that the spectral background intensity and spectral profile have some differences for different kinds of solutions. The spectra of the three kinds of solutions all have strong peak value at 485 and 655 nm, but the intensity ratios between the two peaks are different. Besides, there are characteristic peak at 475, 576 and 630 nm for glucose, and the sucrose has apparent characteristic peak at 632 nm, these characteristic peaks can be used for detecting whether the natural honey has been added glucose or sucrose. By comparing two kinds of spectrum of the same solution, the intensity of photoacoustic spectrum is more responsive to the wavelength, indicating photoacoustic spectrometry has a higher sensitivity in the test of material composition.

摘要

采用单光束光声光谱仪测量了葡萄糖、蔗糖和蜂蜜溶液在可见光范围内的光声光谱,并与分光光度法得到的光谱进行了比较。上述溶液的光谱特性表明,不同种类溶液的光谱背景强度和光谱轮廓存在一些差异。三种溶液的光谱在485和655nm处均有较强的峰值,但两峰之间的强度比不同。此外,葡萄糖在475、576和630nm处有特征峰,蔗糖在632nm处有明显的特征峰,这些特征峰可用于检测天然蜂蜜中是否添加了葡萄糖或蔗糖。通过比较同一种溶液的两种光谱,发现光声光谱的强度对波长更敏感,表明光声光谱法在材料成分检测中具有更高的灵敏度。

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