含他莫昔芬的滴眼液成功在整个眼睛中引发了Cre介导的重组。

Tamoxifen-Containing Eye Drops Successfully Trigger Cre-Mediated Recombination in the Entire Eye.

作者信息

Schlecht Anja, Leimbeck Sarah V, Tamm Ernst R, Braunger Barbara M

机构信息

Institute of Human Anatomy and Embryology, University of Regensburg, Universitätsstr. 31, D-93053, Regensburg, Germany.

出版信息

Adv Exp Med Biol. 2016;854:495-500. doi: 10.1007/978-3-319-17121-0_66.

DOI:10.1007/978-3-319-17121-0_66

PMID:26427451

Abstract

Embryonic lethality in mice with targeted gene deletion is a major issue that can be circumvented by using Cre-loxP-based animal models. Various inducible Cre systems are available, e.g. such that are activated following tamoxifen treatment, and allow deletion of a specific target gene at any desired time point during the life span of the animal. In this study, we describe the efficiency of topical tamoxifen administration by eye drops using a Cre- reporter mouse strain (R26R). We report that tamoxifen-responsive CAGGCre-ER (TM) mice show a robust Cre- mediated recombination throughout the entire eye.

摘要

靶向基因缺失小鼠的胚胎致死性是一个主要问题，可通过使用基于Cre-loxP的动物模型来规避。有多种可诱导的Cre系统，例如在他莫昔芬处理后被激活的系统，这些系统允许在动物寿命期间的任何期望时间点删除特定的靶基因。在本研究中，我们描述了使用Cre报告基因小鼠品系（R26R）通过眼药水局部给予他莫昔芬的效率。我们报告说，对他莫昔芬有反应的CAGGCre-ER（TM）小鼠在整个眼睛中显示出强大的Cre介导的重组。