逆转录病毒载体的纯化与注射
Purification and Injection of Retroviral Vectors.
作者信息
Tashiro Ayumu, Zhao Chunmei, Suh Hoonkyo, Gage Fred H
出版信息
Cold Spring Harb Protoc. 2015 Oct 1;2015(10):925-31. doi: 10.1101/pdb.prot086371.
PMID:26430255
Abstract
Retroviral vectors are powerful tools for genetic manipulation. This protocol discusses the production, purification, and use of replication-deficient retroviral vectors based on Moloney murine leukemia virus and lentivirus. It also describes the injection of a retroviral vector into the dentate gyrus of young adult mice to fluorescently label live murine brain tissue.
摘要
逆转录病毒载体是用于基因操作的强大工具。本方案讨论了基于莫洛尼鼠白血病病毒和慢病毒的复制缺陷型逆转录病毒载体的生产、纯化和使用。它还描述了将逆转录病毒载体注射到成年幼鼠齿状回中以对活的鼠脑组织进行荧光标记的方法。
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