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可变入射角的微衰减全反射傅里叶变换红外(Micro ATR FT-IR)光谱成像

Micro-Attenuated Total Reflection Fourier Transform Infrared (Micro ATR FT-IR) Spectroscopic Imaging with Variable Angles of Incidence.

作者信息

Wrobel Tomasz P, Vichi Alessandra, Baranska Malgorzata, Kazarian Sergei G

机构信息

Jagiellonian Centre for Experimental Therapeutics (JCET), Jagiellonian University, Bobrzynskiego 14, 30-348 Krakow, Poland.

出版信息

Appl Spectrosc. 2015 Oct;69(10):1170-4. doi: 10.1366/15-07963.

Abstract

The control of the angle of incidence in attenuated total reflection (ATR) Fourier transform infrared (FT-IR) spectroscopy allows for the probing of the sample at different depths of penetration of the evanescent wave. This approach has been recently coupled with macro-imaging capability using a diamond ATR accessory. In this paper, the design of optical apertures for the micro-germanium (Ge) ATR objective is presented for an FT-IR spectroscopic imaging microscope, allowing measurements with different angles of incidence. This approach provides the possibility of three-dimensional (3D) profiling in micro-ATR FT-IR imaging mode. The proof of principle results for measurements of polymer laminate samples at different angles of incidence confirm that controlling the depth of penetration is possible using a Ge ATR objective with added apertures.

摘要

在衰减全反射(ATR)傅里叶变换红外(FT-IR)光谱中,通过控制入射角可以在倏逝波的不同穿透深度对样品进行探测。最近,这种方法已与使用金刚石ATR附件的宏观成像能力相结合。本文介绍了用于傅里叶变换红外光谱成像显微镜的微型锗(Ge)ATR物镜的光阑设计,可实现不同入射角的测量。这种方法为微ATR FT-IR成像模式下的三维(3D)轮廓分析提供了可能性。在不同入射角下对聚合物层压样品进行测量的原理验证结果证实,使用带有附加光阑的锗ATR物镜可以控制穿透深度。

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