Neng N R, Ahmad S M, Gaspar H, Nogueira J M F
Centro de Química e Bioquímica, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Centro de Química e Bioquímica, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Talanta. 2015 Nov 1;144:105-9. doi: 10.1016/j.talanta.2015.05.038. Epub 2015 May 21.
Bar adsorptive microextraction combined with liquid desorption followed by high performance liquid chromatography with diode array detection (BAµE-LD/HPLC-DAD) is proposed for the determination of the psychoactive alkaloid mitragynine (MG) in human urine matrices. By using a modified N-vinylpyrrolidone polymer (P2) sorbent phase, high selectivity and efficiency is achieved. Assays performed by BAµE(P2)-LD/HPLC-DAD on 25 mL water samples spiked at the 8.0 µg L(-1) level yielded average recoveries around 100% of MG, under optimized experimental conditions. The analytical performance showed good precision (RSD<15%), appropriated detection limits of 0.10 µg L(-1) and linear dynamic ranges (0.6-24.0 μg L(-1)) with convenient determination coefficients of 0.9924. By using the standard addition method, the application of the present methodology for the determination of MG in human urine matrices after Kratom consumer, allowed very good performances. The proposed methodology proved to be a suitable alternative to monitor MG in biological fluid matrices, showing to be easy to implement, reliable, sensitive and requiring low sample volumes, when compared with other sorbent-based methods.
本文提出了一种采用棒吸附微萃取结合液体解吸，再通过二极管阵列检测高效液相色谱法（BAµE-LD/HPLC-DAD）测定人尿液基质中精神活性生物碱帽柱木碱（MG）的方法。通过使用改性的N-乙烯基吡咯烷酮聚合物（P2）吸附剂相，实现了高选择性和高效率。在优化的实验条件下，采用BAµE(P2)-LD/HPLC-DAD对加标量为8.0 µg L(-1)的25 mL水样进行分析，MG的平均回收率约为100%。分析性能显示出良好的精密度（RSD<15%），0.10 µg L(-1)的适宜检测限和线性动态范围（0.6 - 24.0 μg L(-1)），测定系数为0.9924。通过标准加入法，本方法在测定服用 kratom 后的人尿液基质中MG时表现良好。与其他基于吸附剂的方法相比，所提出的方法被证明是监测生物流体基质中MG的合适替代方法，具有易于实施、可靠、灵敏且所需样品体积小的特点。
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