Tejedor Junco María Teresa, Gonzalez-Martin Margarita, Rodriguez Gonzalez Noe Francisco, Gutierrez Carlos
Institute of Biomedical and Health Research, University of Las Palmas de Gran Canaria, P.O. BOX 550, 35080 Las Palmas de Gran Canaria, Canary Islands, Spain.
Vet Ital. 2015 Jul-Sep;51(3):179-83. doi: 10.12834/VetIt.322.1280.2.
This study investigated the presence of Enterococcus spp. strains in camel faeces, their virulence factors, and resistance to the antibiotics commonly used as therapy of enterococcal infections. One hundred and seventy three Enterococcus strains were isolated and identified to species level using polymerase chain reaction (PCR). Susceptibility to 11 antimicrobials was determined by disk diffusion method. Minimal Inhibitory Concentrations (MIC) of penicillin, ampicillin, vancomycin, teicoplanin, gentamicin, and streptomycin were all determined. Genes encoding resistance to vancomycin, tetracycline, and erythromycin as well as genes encoding some virulence factors were identified by PCR. Enterococcus hirae (54.3%) and Enterococcus faecium (25.4%) were the species most frequently isolated. None of the strains were resistant to vancomycin, teicoplanin, ampicillin or showed high level aminoglycoside resistance (HLAR). Strains resistant to rifampicin (42.42%) were those most commonly found followed those resistant to trimethoprim - sulfamethoxazole (33.33%). The genes tetM, tetL, vanC1, and vanC2-C3 were detected in some strains. Virulence genes were not detected. Monitoring the presence of resistant strains of faecal enterococci in animal used with recreational purposes is important to prevent transmission of those strains to humans and to detect resistance or virulence genes that could be transferred to other clinically important bacteria.
本研究调查了骆驼粪便中肠球菌属菌株的存在情况、其毒力因子以及对常用于治疗肠球菌感染的抗生素的耐药性。使用聚合酶链反应(PCR)分离并鉴定了173株肠球菌至种水平。采用纸片扩散法测定了对11种抗菌药物的敏感性。测定了青霉素、氨苄西林、万古霉素、替考拉宁、庆大霉素和链霉素的最低抑菌浓度(MIC)。通过PCR鉴定了编码对万古霉素、四环素和红霉素耐药的基因以及编码一些毒力因子的基因。平肠球菌(54.3%)和屎肠球菌(25.4%)是最常分离出的菌种。没有菌株对万古霉素、替考拉宁、氨苄西林耐药或表现出高水平氨基糖苷类耐药(HLAR)。对利福平耐药的菌株(42.42%)最为常见,其次是对甲氧苄啶-磺胺甲恶唑耐药的菌株(33.33%)。在一些菌株中检测到tetM、tetL、vanC1和vanC2-C3基因。未检测到毒力基因。监测用于娱乐目的动物粪便中肠球菌耐药菌株的存在情况对于防止这些菌株传播给人类以及检测可能转移到其他临床重要细菌的耐药或毒力基因非常重要。