L-岩藻糖可阻断兔肺泡巨噬细胞由巨噬细胞移动抑制因子/巨噬细胞激活因子引发的对佛波酯诱导的氧化反应。

L-fucose blocks MIF/MAF priming of rabbit alveolar macrophages for a PMA-induced oxidative response.

作者信息

Umehara K, Hayakawa H, Myrvik Q N

机构信息

Department of Microbiology and Immunology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103.

出版信息

Cell Immunol. 1989 Mar;119(1):67-72. doi: 10.1016/0008-8749(89)90224-4.

DOI:10.1016/0008-8749(89)90224-4

PMID:2646027

Abstract

The effect of L-fucose on the priming of AM from normal adult rabbits and their subsequent chemiluminescent (CL) responses to phorbol myristate acetate (PMA) was investigated. It was observed that 12.5 mM L-fucose, but not D-fucose, blocked the "spontaneous" priming of normal AM during an 18-hr incubation period in serum-free RPMI 1640 medium by about 40% (P less than 0.01) as detected by their CL responses to a PMA challenge. In addition, the optimal concentration of L-fucose (12.5 mM) blocked MIF/MAF priming during the 3- or 18-hr incubation period by 71 or 93% (P less than 0.05), respectively, as determined by their CL responses following PMA challenge. It is of particular significance that D-fucose was inactive. These results, together with previously published data, indicate that L-fucose (a) blocks priming of AM for an oxidative response, (b) stimulates random migration of AM, and (c) reverses migration inhibition produced by migration inhibition factor.

摘要

研究了L-岩藻糖对正常成年兔肺泡巨噬细胞（AM）的启动作用及其随后对佛波酯（PMA）的化学发光（CL）反应。观察到，在无血清的RPMI 1640培养基中孵育18小时期间，12.5 mM的L-岩藻糖而非D-岩藻糖，通过其对PMA刺激的CL反应检测，使正常AM的“自发”启动受到约40%的阻断（P<0.01）。此外，L-岩藻糖的最佳浓度（12.5 mM）在3小时或18小时孵育期间，分别通过PMA刺激后的CL反应测定，使巨噬细胞移动抑制因子/巨噬细胞激活因子启动受到71%或93%的阻断（P<0.05）。特别重要的是D-岩藻糖无活性。这些结果与先前发表的数据一起表明，L-岩藻糖（a）阻断AM对氧化反应的启动，（b）刺激AM的随机迁移，以及（c）逆转由迁移抑制因子产生的迁移抑制。