Liu Ying, Liao Mei, He Xueling, Liu Xia, Kou Xingming, Xiao Dan
College of Chemistry, Sichuan University.
Anal Sci. 2015;31(10):971-7. doi: 10.2116/analsci.31.971.
In this paper, nitrogen-doped carbon dots (N-CDs) with high quantum yield (QY) of 40.5% were prepared through a facile and straightforward hydrothermal route. The as-prepared N-CDs exhibited excellent photoluminescence properties, good water-solublity and photostability, negligible cytotoxicity and favourable biocompatibility. Such N-CDs were found to serve as an effective fluorescent sensor for selective and sensitive detection of Hg(2+) in a wide linear response concentration range of 0 - 8 μM with a limit of detection (LOD) of 0.087 μM and could be applied to the determination of Hg(2+) in environmental water samples. The corresponding mechanisms were discussed in detail. Moreover, another attractive finding was that the N-CDs showed satisfactory performance in bioimaging before and after the addition of Hg(2+) in human lung cancer PC14 cells. With excellent sensitivity, selectivity and biocompatibility, such cheap carbonmaterials are potentially suitable for monitoring of Hg(2+) in environmental applications and promising for biological applications.
本文通过简便直接的水热法制备了量子产率高达40.5%的氮掺杂碳点（N-CDs）。所制备的N-CDs表现出优异的光致发光性能、良好的水溶性和光稳定性、可忽略不计的细胞毒性以及良好的生物相容性。发现这种N-CDs可作为一种有效的荧光传感器，用于在0 - 8 μM的宽线性响应浓度范围内选择性和灵敏地检测Hg(2+)，检测限为0.087 μM，并可应用于环境水样中Hg(2+)的测定。详细讨论了相应的机制。此外，另一个引人注目的发现是，在人肺癌PC14细胞中添加Hg(2+)前后，N-CDs在生物成像方面表现出令人满意的性能。由于具有优异的灵敏度、选择性和生物相容性，这种廉价的碳材料在环境应用中潜在地适用于Hg(2+)的监测，并且在生物应用方面前景广阔。
