Gao Xing, Wang Li, Huang Hai-Liang, Wang Lin-Lin, Yao Jun-Liang, Shi Shuo, Yao Tian-Ming
Shanghai Key Lab of Chemical Assessment and Sustainability, Department of Chemistry, Tongji University, 1239 Siping Road, 200092, Shanghai, PR China.
Analyst. 2015 Nov 21;140(22):7513-7. doi: 10.1039/c5an01624j.
Monitoring the aggregation of the tau protein is a key protocol for elucidating the pathogenic mechanism of Alzheimer's disease. In the present article, Ru(phen)2dppzidzo, a "light switch" ruthenium(ii) complex, was presented as a new monitoring probe for the aggregation of a tau R3 peptide, the third repeat unit of the tau microtubule-binding domain. Having little impact on the aggregation process, large fixed Stokes shift and small background luminescence made the complex a better probe for monitoring the aggregation process and quantitatively detecting tau filaments compared to thioflavin S, a commonly used fluorescent dye for staining neurofibrillary tangles and monitoring tau aggregation. Furthermore, a long luminescence lifetime of this complex could also expand its potential usage in the detection of tau filaments in the presence of short-lived fluorescent backgrounds.
监测tau蛋白的聚集是阐明阿尔茨海默病致病机制的关键方案。在本文中，Ru(phen)2dppzidzo，一种“光开关”钌(ii)配合物，被提出作为tau R3肽（tau微管结合结构域的第三个重复单元）聚集的新型监测探针。该配合物对聚集过程影响小、斯托克斯位移大且背景发光小，与硫黄素S（一种常用于染色神经原纤维缠结和监测tau聚集的荧光染料）相比，使其成为监测聚集过程和定量检测tau细丝的更好探针。此外，该配合物的长发光寿命还可扩大其在存在短寿命荧光背景时检测tau细丝的潜在用途。
