Rho激酶抑制剂Y27632对猪角膜内皮细胞培养、炎症及免疫调节的影响
Effect of Rho-kinase Inhibitor, Y27632, on Porcine Corneal Endothelial Cell Culture, Inflammation and Immune Regulation.
作者信息
Lee Whayoung, Miyagawa Yuko, Long Cassandra, Zhang Matthew, Cooper David K C, Hara Hidetaka
机构信息
a Thomas E. Starzl Transplantation Institute, Department of Surgery, University of Pittsburgh , Pittsburgh , Pennsylvania , USA.
出版信息
Ocul Immunol Inflamm. 2016 Oct;24(5):579-93. doi: 10.3109/09273948.2015.1056534. Epub 2015 Oct 16.
PURPOSE
To investigate the effect of the Rho-kinase inhibitor, Y27632, on pig corneal endothelial cell (pCEC) culture, and on inflammation and immune regulation of the responses of human cells to pCECs.
METHODS
pCECs were cultured with/without Y27632 to assess cell proliferation and in vitro wound healing assay. The level of MCP-1 and VEGF in pCECs stimulated with human TNF-α were measured. Proliferation of human PBMCs stimulated with pCECs, and cytokine production in human T cells, and monocyte migration after stimulation were investigated.
RESULTS
Y27632 promoted pCEC proliferation, prevented pCEC death, and enhanced in vitro wound healing. After stimulation, there were significantly lower levels of MCP-1 and VEGF measured in pCECs cultured with Y27632, and significantly reduced human PBMC proliferation, cytokine production, and monocyte migration.
CONCLUSIONS
The application of the Rho-kinase inhibitor will be beneficial when culturing pCECs, and may provide a novel therapy to reduce inflammation after corneal xenotransplantation.
目的
研究Rho激酶抑制剂Y27632对猪角膜内皮细胞（pCEC）培养的影响，以及对人细胞对pCEC反应的炎症和免疫调节作用。
方法
将pCEC在有/无Y27632的条件下培养，以评估细胞增殖和体外伤口愈合试验。检测用人肿瘤坏死因子-α刺激的pCEC中MCP-1和VEGF的水平。研究用pCEC刺激的人外周血单个核细胞（PBMC）的增殖、人T细胞中的细胞因子产生以及刺激后的单核细胞迁移。
结果
Y27632促进pCEC增殖，防止pCEC死亡，并增强体外伤口愈合。刺激后，在用Y27632培养的pCEC中测得的MCP-1和VEGF水平显著降低，人PBMC增殖、细胞因子产生和单核细胞迁移显著减少。
结论
Rho激酶抑制剂的应用在培养pCEC时将是有益的，并且可能为减少角膜异种移植后的炎症提供一种新的治疗方法。
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