Hollywood M A
Ir J Med Sci. 2016 Feb;185(1):1-10. doi: 10.1007/s11845-015-1362-x.
The Conway Review Lecture is held annually by the Royal Academy of Medicine in Ireland Biomedical Sciences Section, to remember the life and scientific work of a world class Irish scientist, Professor Edward J Conway.
This years lecture will focus on large conductance Ca2+ activated K+ (BK) channels and aims to describe how a combination of techniques can be used to unravel drug effects on ion channels at a molecular level.
Experiments were performed using a range of techniques including patch clamp electrophysiology, mutagenesis, structural biology and mathematical modeling.
Our data suggest that the novel BK channel opener GoSlo-SR-5-6 mediates its effects via an interaction with 2 residues on S6 (S317 and I326) and a residue on the S4/S5 linker (L227).
We hypothesize that this novel opener activates BK channels by altering an interaction between the S4/S5 linker and the pore-forming S6 transmembrane helix.
康威纪念讲座由爱尔兰皇家医学院生物医学科学部每年举办一次，以缅怀世界级爱尔兰科学家爱德华·J·康威教授的生平与科学成就。
今年的讲座将聚焦于大电导钙激活钾（BK）通道，旨在描述如何结合多种技术在分子水平上揭示药物对离子通道的作用。
采用了一系列技术进行实验，包括膜片钳电生理学、诱变、结构生物学和数学建模。
我们的数据表明，新型BK通道开放剂GoSlo-SR-5-6通过与S6上的两个残基（S317和I326）以及S4/S5连接子上的一个残基（L227）相互作用来介导其效应。
我们推测这种新型开放剂通过改变S4/S5连接子与形成孔道的S6跨膜螺旋之间的相互作用来激活BK通道。
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