Lefsih Khalef, Delattre Cédric, Pierre Guillaume, Michaud Philippe, Aminabhavi Tejraj M, Dahmoune Farid, Madani Khodir
Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria; Département de Biochimie et Microbiologie, Faculté des Sciences Biologiques et des Sciences Agronomiques, Université de Tizi ouzou, 15000 Tizi ouzou, Algeria.
Clermont Université, Université Blaise Pascal, Institut Pascal, UMR CNRS 6602 CNRS Polytech Clermont-Ferrand, 24 avenue des Landais, BP 206, Aubière Cedex F-63174, France.
Int J Biol Macromol. 2016 Jan;82:645-52. doi: 10.1016/j.ijbiomac.2015.10.046. Epub 2015 Oct 19.
Total Pectins Fraction (TPF) was extracted at room temperature from dried cladodes of Opuntia ficus indica. TPF is constituted of three pectic fractions WSP, CSP and ASP, which are made up of 66.6%, 44.3% and 81.1% (w/w) of galacturonic acid, respectively. The antioxidant ability of TPF increased with the concentration increasing. It scavenged hydroxyl radical by 90% and chelated 90% of ferrous ions at 5 g/L. FTIR study was carried out. Strong characteristic absorption peaks at 1,618 cm(-1) assigned to the vibration of COO(-) group of galacturonic acid. In the fingerprint region, we noticed three well-defined peaks at 1054, 1085, and 1,154 cm(-1) characteristic of pectic polysaccharides. TPF are non-gelling pectins. The co-crosslinking of TPF with carrageenan was carried out and the gelling behavior was successfully improved. Thermo-sensitive hydrogel was obtained with 82% of TPF and 18% of carrageenan (w/w).
从干燥的仙人掌肉质茎中在室温下提取总果胶组分（TPF）。TPF由三种果胶组分水溶性果胶（WSP）、柠檬酸溶性果胶（CSP）和碱溶性果胶（ASP）组成，它们分别由66.6%、44.3%和81.1%（w/w）的半乳糖醛酸组成。TPF的抗氧化能力随浓度增加而增强。在5 g/L时，它对羟基自由基的清除率为90%，对亚铁离子的螯合率为90%。进行了傅里叶变换红外光谱（FTIR）研究。在1618 cm⁻¹处有强特征吸收峰，归属于半乳糖醛酸COO⁻基团的振动。在指纹区，我们注意到在1054、1085和1154 cm⁻¹处有三个明确的峰，这是果胶多糖的特征峰。TPF是非胶凝性果胶。进行了TPF与卡拉胶的共交联，成功改善了其胶凝行为。用82%的TPF和18%的卡拉胶（w/w）获得了热敏水凝胶。
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