16S rRNA基因测序在确诊疑似新生儿败血症中的作用
The Role of 16S rRNA Gene Sequencing in Confirmation of Suspected Neonatal Sepsis.
作者信息
El Gawhary Somaia, El-Anany Mervat, Hassan Reem, Ali Doaa, El Gameel El Qassem
机构信息
*Department of Clinical Pathology, Fayoum University.
**Department of Clinical Pathology, Cairo University.
出版信息
J Trop Pediatr. 2016 Feb;62(1):75-80. doi: 10.1093/tropej/fmv066. Epub 2015 Oct 22.
Different molecular assays for the detection of bacterial DNA in the peripheral blood represented a diagnostic tool for neonatal sepsis. We targeted to evaluate the role of 16S rRNA gene sequencing to screen for bacteremia to confirm suspected neonatal sepsis (NS) and compare with risk factors and septic screen testing. Sixty-two neonates with suspected NS were enrolled. White blood cells count, I/T ratio, C-reactive protein, blood culture and 16S rRNA sequencing were performed. Blood culture was positive in 26% of cases, and PCR was positive in 26% of cases. Evaluation of PCR for the diagnosis of NS showed sensitivity 62.5%, specificity 86.9%, PPV 62.5%, NPV 86.9% and accuracy of 79.7%. 16S rRNA PCR increased the sensitivity of detecting bacterial DNA in newborns with signs of sepsis from 26 to 35.4%, and its use can be limited to cases with the most significant risk factors and positive septic screen.
用于检测外周血中细菌DNA的不同分子检测方法是新生儿败血症的一种诊断工具。我们旨在评估16S rRNA基因测序在筛查菌血症以确诊疑似新生儿败血症（NS）中的作用，并与危险因素和败血症筛查检测进行比较。纳入了62例疑似NS的新生儿。进行了白细胞计数、I/T比值、C反应蛋白、血培养和16S rRNA测序。26%的病例血培养呈阳性，26%的病例PCR呈阳性。评估PCR诊断NS的敏感性为62.5%，特异性为86.9%，阳性预测值为62.5%，阴性预测值为86.9%，准确率为79.7%。16S rRNA PCR将有败血症体征新生儿中细菌DNA检测的敏感性从26%提高到35.4%，其应用可限于具有最显著危险因素且败血症筛查呈阳性的病例。
Zotero 插件