伪对映体混合碳酸酯作为对映选择性筛选有效荧光探针的评估
Evaluation of Pseudoenantiomeric Mixed Carbonates as Efficient Fluorogenic Probes for Enantioselectivity Screening.
作者信息
Zadlo Anna, Koszelewski Dominik, Borys Filip, Ostaszewski Ryszard
机构信息
Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
出版信息
Chembiochem. 2016 Jan 1;17(1):71-6. doi: 10.1002/cbic.201500509. Epub 2015 Nov 23.
We report mixed carbonates as enzyme substrates and demonstrate their application as fluorogenic probes for lipase and esterase enantiopreference screening. By the application of pseudoenantiomers with distinct fluorescence behaviors, it is possible to evaluate the activity and enantiopreference of hydrolytic enzymes. In order to validate our method, enantioselectivities calculated from fluorometric measurements were compared with the results obtained from larger-scale kinetic resolution.
我们报道了混合碳酸盐作为酶底物，并展示了它们作为荧光探针用于脂肪酶和酯酶对映体选择性筛选的应用。通过应用具有不同荧光行为的假对映体，可以评估水解酶的活性和对映体选择性。为了验证我们的方法，将荧光测量计算得到的对映体选择性与大规模动力学拆分得到的结果进行了比较。
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