Su Mi, Qiu Li, Wang Qian, Jiang Zhen, Liu Xiao Juan, Lin Jia, Fang Ding Zhi
Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, PR China.
Ann Nutr Metab. 2015;67(4):236-42. doi: 10.1159/000440777. Epub 2015 Oct 20.
BACKGROUND/AIMS: The association of preproghrelin Leu72Met polymorphism with plasma lipids profile was inconsistently reported and needs more studies to be confirmed. Our study was to investigate the changes of plasma lipids ratios after a high-carbohydrate (high-CHO) diet in healthy Chinese adolescents with different genotypes of this polymorphism.
Fifty-three healthy university students were given a washout diet of 54.1% carbohydrate for 7 days, followed by a high-CHO diet of 70.1% carbohydrate for 6 days. The anthropometric and biological parameters were analyzed at baseline and before and after the high-CHO diet.
When compared with those before the high-CHO diet, body mass index (BMI) decreased in the male and female Met72 allele carriers. Decreased low-/high-density lipoprotein cholesterol (LDL-C/HDL-C) was observed in all participants except the female subjects with the Leu72Leu genotype. TG/HDL-C and log (TG/HDL-C) were increased only in the female subjects with the Leu72Leu genotype.
These results suggest that the Met72 allele of preproghrelin Leu72Met polymorphism may be associated with decreased BMI induced by the high-CHO diet in male and female adolescents, while the Leu72 allele with increased TG/HDL-C and log (TG/HDL-C) in the female adolescents only. Furthermore, the decreasing effect of the high-CHO diet on LDL/HDL-C may be eliminated in the female Leu72Leu homozygotes.
背景/目的：关于前胃泌素释放肽Leu72Met多态性与血脂谱的关联报道并不一致，需要更多研究来证实。我们的研究旨在调查不同基因型的健康中国青少年在高碳水化合物（高CHO）饮食后血脂比例的变化。
53名健康大学生先接受7天含54.1%碳水化合物的洗脱饮食，随后接受6天含70.1%碳水化合物的高CHO饮食。在基线以及高CHO饮食前后分析人体测量和生物学参数。
与高CHO饮食前相比，男性和女性Met72等位基因携带者的体重指数（BMI）下降。除Leu72Leu基因型的女性受试者外，所有参与者的低密度/高密度脂蛋白胆固醇（LDL-C/HDL-C）均下降。仅Leu72Leu基因型的女性受试者的TG/HDL-C和log（TG/HDL-C）升高。
这些结果表明，前胃泌素释放肽Leu72Met多态性的Met72等位基因可能与高CHO饮食诱导的男性和女性青少年BMI下降有关，而Leu72等位基因仅与女性青少年TG/HDL-C和log（TG/HDL-C）升高有关。此外，高CHO饮食对LDL/HDL-C的降低作用在女性Leu72Leu纯合子中可能会消除。
