白细胞介素-4的组成性分泌促使CD133+侧群细胞抵抗药物治疗和细胞死亡。
Constitutive secretion of Interleukin-4 dictates CD133+ side population cells to resist drug treatment and cell death.
作者信息
Guan Guo-Fang, Tang Xu-Xia, Zhang De-Jun, Zheng Ying, Yu Duo-Jiao, Zhao Yan, Lu Yan-Qing, Zhu Lin
机构信息
Department of Otolaryngology, Head and Neck Surgery. The Second Hospital of Jilin University, Changchun 130041, China.
出版信息
J BUON. 2015 Sep-Oct;20(5):1350-9.
PURPOSE
In the present study, we made an attempt to elucidate the role of oversecretion of interleukin-4 (IL-4) in cancer stem cells (CSCs) of head and neck squamous cell carcinoma (HNSCC).
METHODS
HNSCC samples were analyzed for the presence of CSCs by flow cytometry. In addition, we have performed drug and apoptosis resistance assays to determine the role of IL-4 in CSCs.
RESULTS
HNSCC samples contained 3.3% of CD133+ cancer stem like side population (SP) cells in HNSCC which displayed infinite cell proliferation and they had high self-renewal capacity. These CD133+ cells displayed enhanced expression of IL-4, which promoted multidrug and apoptosis resistance. After neutralizing IL-4, the CD133+ SP cells became more sensitive to drug treatment and apoptosis.
CONCLUSIONS
Our data suggest that the autocrine secretion of IL-4 is a potential target for the development of novel anticancer drugs to prevent the CSCs-mediated therapy failure and tumorigenesis.
目的
在本研究中，我们试图阐明白细胞介素-4（IL-4）过度分泌在头颈部鳞状细胞癌（HNSCC）癌干细胞（CSCs）中的作用。
方法
通过流式细胞术分析HNSCC样本中CSCs的存在情况。此外，我们进行了药物和凋亡抗性测定，以确定IL-4在CSCs中的作用。
结果
HNSCC样本中含有3.3%的CD133+癌干细胞样侧群（SP）细胞，这些细胞表现出无限的细胞增殖能力且具有高自我更新能力。这些CD133+细胞IL-4表达增强，促进了多药抗性和凋亡抗性。中和IL-4后，CD133+ SP细胞对药物治疗和凋亡变得更加敏感。
结论
我们的数据表明，IL-4的自分泌是开发新型抗癌药物以防止CSCs介导的治疗失败和肿瘤发生的潜在靶点。
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