[局部应用脂联素重组腺病毒对骨缺损修复的影响]
[Effect of topically applied recombinant adenovirus of adiponectin on bone defect repair].
作者信息
Lü Xue, Liu Na, Du Wen, Li Jiayang, Sun Yue, Luo En
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2015 Aug;33(4):361-5. doi: 10.7518/hxkq.2015.04.008.
OBJECTIVE
To investigate the effect of local administration of recombinant adenovirus of human adiponectin (hAPN) Ad-hAPN-EGFP on tibial defect repair of SD rats.
METHODS
Tibial defect (2 mm) models of 36 SD rats (72 sides) were randomly divided into three groups (A, B, and C; n=24). The three groups were injected with Ad-hAPN-EGFP, Ad-EGFP, and normal saline, respectively, during operation and the day after operation. A week after the operation, the expression of hAPN and osteogenesis-related factors were detected by real-time polymerase chain reaction. Three weeks after operation, the tibias were examined by micro-computed tomography, hematoxylin-eosin staining, and Masson staining to evaluate the restoration of bone defects.
RESULTS
- The hAPN expression was detected in group A but not in groups B and C. Osteogenesis-related factors expression of group A was significantly higher than that of the other groups (P<0.05). 2) Osteogenesis (including bone mineral density, relative bone volume, trabecular number and trabecular thickness) in group A were more evident than those in groups B and C (P<0.05). No significant differences were found between groups B and C (P>0.05).
CONCLUSION
Local administration of recombinant adenovirus Ad-hAPN-EGFP may be an effective strategy to improve the restoration of bone defects in vivo.
目的
探讨局部注射重组人脂联素腺病毒（hAPN）Ad-hAPN-EGFP对SD大鼠胫骨缺损修复的影响。
方法
将36只SD大鼠（72侧）的胫骨缺损（2mm）模型随机分为三组（A组、B组和C组；n = 24）。三组分别在手术中和术后当天注射Ad-hAPN-EGFP、Ad-EGFP和生理盐水。术后1周，通过实时聚合酶链反应检测hAPN及成骨相关因子的表达。术后3周，对胫骨进行显微计算机断层扫描、苏木精-伊红染色和Masson染色，以评估骨缺损的修复情况。
结果
1）A组检测到hAPN表达，B组和C组未检测到。A组成骨相关因子的表达明显高于其他组（P<0.05）。2）A组的成骨情况（包括骨密度、相对骨体积、骨小梁数量和骨小梁厚度）比B组和C组更明显（P<0.05）。B组和C组之间无显著差异（P>0.05）。
结论
局部注射重组腺病毒Ad-hAPN-EGFP可能是一种改善体内骨缺损修复的有效策略。
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