原代和肿瘤性肝细胞中四跨膜蛋白相关磷酸肌醇-4激酶的测定与表征

Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells.

作者信息

Rombouts Krista, Carloni Vinicio

机构信息

Division of Medicine, Institute for Liver & Digestive Health, Royal Free Hospital, University College London (UCL), Royal Free Campus, Rowland Hill Street, London, NW3 2PF, UK.

Department of Experimental and Clinical Medicine, Center for Research, Transfer and High Education, DENOthe, University of Florence, Largo Brambilla 3, Florence, 50134, Italy.

出版信息

Methods Mol Biol. 2016;1376:203-12. doi: 10.1007/978-1-4939-3170-5_17.

DOI:10.1007/978-1-4939-3170-5_17

PMID:26552686

Abstract

Accumulating evidence implicates phosphoinositide 4-phosphate as a regulatory molecule in its own right recruiting specific effector proteins to cellular membranes. Here, we describe biochemical and immunocytochemical methods to evaluate tetraspanin-associated phosphoinositide-4 kinases activity in primary human hepatic stellate cells (hHSC) and neoplastic hepatoblastoma cells.

摘要

越来越多的证据表明，磷脂酰肌醇4-磷酸本身就是一种调节分子，可将特定效应蛋白招募到细胞膜上。在此，我们描述了生化和免疫细胞化学方法，以评估原代人肝星状细胞（hHSC）和肿瘤性肝母细胞瘤细胞中四跨膜蛋白相关的磷脂酰肌醇-4激酶活性。