皮下和内脏脂肪组织中类固醇硫酸酯酶活性：绝经前后妇女的比较。
Steroid sulfatase activity in subcutaneous and visceral adipose tissue: a comparison between pre- and postmenopausal women.
机构信息
Folkhälsan Research CenterBiomedicum Helsinki, 00290 Helsinki, FinlandUniversity of Helsinki and Helsinki University Central HospitalHeart and Lung Center, Helsinki, FinlandUniversity of Helsinki and Helsinki University Central HospitalObstetrics and Gynecology, Helsinki, FinlandHelsinki University Central HospitalHUSLAB, Helsinki, FinlandNational Institute for Health and WelfareGenomics and Biomarkers Unit, Helsinki, Finland Folkhälsan Research CenterBiomedicum Helsinki, 00290 Helsinki, FinlandUniversity of Helsinki and Helsinki University Central HospitalHeart and Lung Center, Helsinki, FinlandUniversity of Helsinki and Helsinki University Central HospitalObstetrics and Gynecology, Helsinki, FinlandHelsinki University Central HospitalHUSLAB, Helsinki, FinlandNational Institute for Health and WelfareGenomics and Biomarkers Unit, Helsinki, Finland
Folkhälsan Research CenterBiomedicum Helsinki, 00290 Helsinki, FinlandUniversity of Helsinki and Helsinki University Central HospitalHeart and Lung Center, Helsinki, FinlandUniversity of Helsinki and Helsinki University Central HospitalObstetrics and Gynecology, Helsinki, FinlandHelsinki University Central HospitalHUSLAB, Helsinki, FinlandNational Institute for Health and WelfareGenomics and Biomarkers Unit, Helsinki, Finland Folkhälsan Research CenterBiomedicum Helsinki, 00290 Helsinki, FinlandUniversity of Helsinki and Helsinki University Central HospitalHeart and Lung Center, Helsinki, FinlandUniversity of Helsinki and Helsinki University Central HospitalObstetrics and Gynecology, Helsinki, FinlandHelsinki University Central HospitalHUSLAB, Helsinki, FinlandNational Institute for Health and WelfareGenomics and Biomarkers Unit, Helsinki, Finland.
出版信息
Eur J Endocrinol. 2016 Feb;174(2):167-75. doi: 10.1530/EJE-15-0831. Epub 2015 Nov 9.
OBJECTIVE
Adipose tissue is an important extragonadal site for steroid hormone biosynthesis. After menopause, estrogens are synthesized exclusively in peripheral tissues from circulating steroid precursors, of which the most abundant is dehydroepiandrosterone sulfate (DHEAS). Our aim was to study activity of steroid sulfatase, an enzyme hydrolyzing DHEAS, and expression of steroid-converting enzyme genes in subcutaneous and visceral adipose tissue derived from pre- and postmenopausal women.
DESIGN
Serum and paired abdominal subcutaneous and visceral adipose tissue samples were obtained from 18 premenopausal and seven postmenopausal women undergoing elective surgery for non-malignant reasons in Helsinki University Central Hospital.
METHODS
To assess steroid sulfatase activity, radiolabeled DHEAS was incubated in the presence of adipose tissue homogenate and the liberated dehydroepiandrosterone (DHEA) was measured. Gene mRNA expressions were analyzed by quantitative RT-PCR. Serum DHEAS, DHEA, and estrogen concentrations were determined by liquid chromatography-tandem mass spectrometry.
RESULTS
Steroid sulfatase activity was higher in postmenopausal compared to premenopausal women in subcutaneous (median 379 vs 257 pmol/kg tissue per hour; P=0.006) and visceral (545 vs 360 pmol/kg per hour; P=0.004) adipose tissue. Visceral fat showed higher sulfatase activity than subcutaneous fat in premenopausal (P=0.035) and all (P=0.010) women. The mRNA expression levels of two estradiol-producing enzymes, aromatase and 17β-hydroxysteroid dehydrogenase type 12, were higher in postmenopausal than in premenopausal subcutaneous adipose tissue.
CONCLUSIONS
Steroid sulfatase activity in adipose tissue was higher in postmenopausal than in premenopausal women suggesting that DHEAS, derived from the circulation, could be more efficiently utilized in postmenopausal adipose tissue for the formation of biologically active sex hormones.
目的
脂肪组织是类固醇激素生物合成的一个重要的性腺外部位。绝经后，雌激素仅在外周组织中由循环类固醇前体合成，其中最丰富的是硫酸脱氢表雄酮（DHEAS）。我们的目的是研究水解 DHEAS 的酶——类固醇硫酸酯酶的活性以及来自绝经前和绝经后妇女的皮下和内脏脂肪组织中类固醇转化酶基因的表达。
设计
从赫尔辛基大学中心医院因非恶性原因接受择期手术的 18 名绝经前和 7 名绝经后妇女获得血清和配对的腹部皮下和内脏脂肪组织样本。
方法
为了评估类固醇硫酸酯酶的活性，用放射性标记的 DHEAS 在脂肪组织匀浆存在的情况下孵育，并测量释放的脱氢表雄酮（DHEA）。通过定量 RT-PCR 分析基因 mRNA 表达。通过液相色谱-串联质谱法测定血清 DHEAS、DHEA 和雌激素浓度。
结果
绝经后妇女的皮下（中位数 379 比 257 pmol/kg 组织/小时；P=0.006）和内脏（545 比 360 pmol/kg 组织/小时；P=0.004）脂肪组织中的类固醇硫酸酯酶活性高于绝经前妇女。在绝经前（P=0.035）和所有（P=0.010）妇女中，内脏脂肪的硫酸酯酶活性均高于皮下脂肪。绝经后妇女的皮下脂肪中，两种产生雌二醇的酶——芳香酶和 17β-羟类固醇脱氢酶 12 的 mRNA 表达水平高于绝经前妇女。
结论
脂肪组织中的类固醇硫酸酯酶活性在绝经后妇女中高于绝经前妇女，这表明来自循环的 DHEAS 可以更有效地用于绝经后脂肪组织中生物活性性激素的形成。
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