F1 -ATP合酶α亚基：一种用于东亚飞蝗RNA干扰介导害虫治理的潜在靶点。
F1 -ATP synthase α-subunit: a potential target for RNAi-mediated pest management of Locusta migratoria manilensis.
作者信息
Hu Jun, Xia Yuxian
机构信息
Genetic Engineering Research Centre, College of Life Science, Chongqing University, Chongqing, China.
Chongqing Engineering Research Centre for Fungal Insecticide, Chongqing, China.
出版信息
Pest Manag Sci. 2016 Jul;72(7):1433-9. doi: 10.1002/ps.4185. Epub 2015 Dec 18.
BACKGROUND
The migratory locust is one of the most destructive agricultural pests worldwide. ATP synthase (F0 F1 -ATPase) uses proton or sodium motive force to produce 90% of the cellular ATP, and the α-subunit of F1 -ATP synthase (ATP5A) is vital for F1 -ATP synthase. Here, we tested whether ATP5A could be a potential target for RNAi-mediated pest management of L. migratoria.
RESULTS
Lm-ATP5A was cloned and characterised. Lm-ATP5A is expressed in all tissues. Injection of 100 ng of the double-stranded RNA of ATP5A (dsATP5A) knocked down the transcription of the target gene and caused mortality in 1.5-5 days. The Lm-ATP5A protein level, the oligomycin-sensitive ATP synthetic and hydrolytic activities and the ATP content were correspondingly reduced following dsATP5A injection.
CONCLUSION
These findings demonstrated the essential roles of Lm-ATP5A in L. migratoria and identified it as a potential target for insect pest control. © 2015 Society of Chemical Industry.
背景
飞蝗是全球最具破坏力的农业害虫之一。ATP合酶（F0F1 -ATP酶）利用质子或钠动力势产生细胞中90%的ATP，而F1 -ATP合酶的α亚基（ATP5A）对F1 -ATP合酶至关重要。在此，我们测试了ATP5A是否可能成为RNA干扰介导的飞蝗害虫治理的潜在靶点。
结果
克隆并鉴定了Lm-ATP5A。Lm-ATP5A在所有组织中均有表达。注射100 ng的ATP5A双链RNA（dsATP5A）可降低靶基因的转录，并在1.5至5天内导致死亡。注射dsATP5A后，Lm-ATP5A蛋白水平、对寡霉素敏感的ATP合成及水解活性以及ATP含量相应降低。
结论
这些发现证明了Lm-ATP5A在飞蝗中的重要作用，并将其确定为害虫防治的潜在靶点。© 2015化学工业协会。
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