来自德国古滕贝格健康研究的血浆同型精氨酸参考区间。

Reference intervals of plasma homoarginine from the German Gutenberg Health Study.

出版信息

Clin Chem Lab Med. 2016 Jul 1;54(7):1231-7. doi: 10.1515/cclm-2015-0785.

DOI:10.1515/cclm-2015-0785

PMID:26562034

Abstract

BACKGROUND

Low circulating homoarginine has been associated with adverse cardiovascular (CV) outcome and mortality in patients at risk and in the general population. The present study aimed to define plasma homoarginine reference intervals from a representative population sample to improve risk stratification between healthy individuals and individuals at risk.

METHODS

We determined age- and sex-specific reference intervals for circulating plasma homoarginine in a subgroup of 786 healthy participants (no CV disease or risk factors) of the Gutenberg Health Study. Homoarginine concentrations were measured using a validated liquid chromatography-tandem mass spectrometry method.

RESULTS

Median EDTA plasma homoarginine concentration was 1.88 [25th; 75th percentile, 1.47; 2.41] μmol/L, with lower concentrations in women (1.77 [1.38; 2.26] μmol/L) than in men (2.01 [1.61; 2.56] μmol/L; p<0.001). Sex-specific 2.5th and 97.5th percentiles of reference intervals were 0.84 and 3.89 μmol/L in women and 0.98 and 4.10 μmol/L in men, respectively. Homoarginine concentrations also depended on age and single nucleotide polymorphisms related to the L-arginine:glycine amidinotransferase gene.

CONCLUSIONS

We provide plasma homoarginine reference intervals in men and women of the general population. The determination of homoarginine levels might be favorable for individual risk stratification.

摘要

背景

低循环同型瓜氨酸与处于危险中的患者和一般人群的不良心血管（CV）结局和死亡率相关。本研究旨在从代表性人群样本中定义血浆同型瓜氨酸的参考区间，以改善健康个体和高危个体之间的风险分层。

方法

我们在哥廷根健康研究的 786 名健康参与者（无 CV 疾病或危险因素）的亚组中确定了循环血浆同型瓜氨酸的年龄和性别特异性参考区间。使用经过验证的液相色谱-串联质谱法测量同型瓜氨酸浓度。

结果

EDTA 血浆同型瓜氨酸浓度的中位数为 1.88 [25 百分位；75 百分位，1.47；2.41] μmol/L，女性（1.77 [1.38；2.26] μmol/L）的浓度低于男性（2.01 [1.61；2.56] μmol/L；p<0.001）。参考区间的性别特异性 2.5 百分位和 97.5 百分位分别为女性 0.84 和 3.89 μmol/L，男性 0.98 和 4.10 μmol/L。同型瓜氨酸浓度也取决于年龄和与 L-精氨酸：甘氨酸酰胺转移酶基因相关的单核苷酸多态性。