使用大孔树脂从山楂叶提取物中分离牡荆素-4″-O-葡萄糖苷和牡荆素-2″-O-鼠李糖苷。

Separation of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves extracts using macroporous resins.

作者信息

Li Hongjuan, Liu Ying, Jin Haizhu, Liu Sujing, Fang Shengtao, Wang Chunhua, Xia Chuanhai

机构信息

Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; School of Pharmacy, Binzhou Medical University, Yantai 264003, PR China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, PR China.

Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Dec 15;1007:23-9. doi: 10.1016/j.jchromb.2015.10.043. Epub 2015 Oct 30.

DOI:10.1016/j.jchromb.2015.10.043

PMID:26562804

Abstract

Vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside are the major flavonoids of hawthorn leaves. In this work, the adsorption and desorption characteristics of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside on seven macroporous resins were evaluated. Among the tested resins, the HPD-400 resin showed the best adsorption and desorption capacities. Adsorption isotherms were constructed for the HPD-400 resin and well fitted to Langmuir and Freundlich models. Dynamic adsorption and desorption tests were performed on column packed with the HPD-400 resin to optimize the chromatographic parameters. After one run treatment with the HPD-400 resin, the contents of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside in the product were increased 8.44-fold and 8.43-fold from 0.720% and 2.63% to 6.08% and 22.2% with recovery yields of 79.1% and 81.2%, respectively. These results show that the developed method is a promising basis for the large-scale purification of vitexin-4″-O-glucoside and vitexin-2″-O-rhamnoside from hawthorn leaves and other plant materials.

摘要

牡荆素-4″-O-葡萄糖苷和牡荆素-2″-O-鼠李糖苷是山楂叶中的主要黄酮类化合物。在本研究中，评估了牡荆素-4″-O-葡萄糖苷和牡荆素-2″-O-鼠李糖苷在七种大孔树脂上的吸附和解吸特性。在所测试的树脂中，HPD-400树脂表现出最佳的吸附和解吸能力。构建了HPD-400树脂的吸附等温线，并与Langmuir和Freundlich模型拟合良好。对填充HPD-400树脂的柱子进行动态吸附和解吸试验，以优化色谱参数。用HPD-400树脂进行一次运行处理后，产品中牡荆素-4″-O-葡萄糖苷和牡荆素-2″-O-鼠李糖苷的含量分别从0.720%和2.63%提高到6.08%和22.2%，回收率分别为79.1%和81.2%。这些结果表明，所开发的方法为从山楂叶和其他植物材料中大规模纯化牡荆素-4″-O-葡萄糖苷和牡荆素-2″-O-鼠李糖苷提供了有前景的基础。